Which of the following is NOT a component of a typical PCR reaction?
Practice Questions
Q1
Which of the following is NOT a component of a typical PCR reaction?
Template DNA
Primers
Nucleotides
RNA polymerase
Questions & Step-by-Step Solutions
Which of the following is NOT a component of a typical PCR reaction?
Step 1: Understand what PCR (Polymerase Chain Reaction) is. It is a method used to make many copies of a specific DNA segment.
Step 2: Identify the main components needed for a typical PCR reaction. These include: DNA template, primers, nucleotides, and a DNA polymerase enzyme.
Step 3: Recognize that RNA polymerase is an enzyme that synthesizes RNA from a DNA template, but it is not used in PCR.
Step 4: Learn that Taq polymerase is the specific enzyme used in PCR for DNA synthesis, not RNA polymerase.
Step 5: Conclude that since RNA polymerase is not part of a typical PCR reaction, it is the correct answer to the question.
