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What are the three main steps of PCR?
Practice Questions
Q1
What are the three main steps of PCR?
Denaturation, annealing, extension
Denaturation, transcription, translation
Ligation, denaturation, amplification
Amplification, sequencing, visualization
Questions & Step-by-Step Solutions
What are the three main steps of PCR?
Steps
Concepts
Step 1: Denaturation - The double-stranded DNA is heated to separate it into two single strands.
Step 2: Annealing - The temperature is lowered to allow short DNA primers to attach to the single strands.
Step 3: Extension - The temperature is raised again so that a special enzyme can add new DNA nucleotides to the primers, creating new strands of DNA.
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