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What are the three main steps of PCR?

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Question: What are the three main steps of PCR?

Options:

  1. Denaturation, annealing, extension
  2. Denaturation, transcription, translation
  3. Ligation, denaturation, amplification
  4. Amplification, sequencing, visualization

Correct Answer: Denaturation, annealing, extension

Solution: 

The three main steps of PCR are denaturation, annealing, and extension.

What are the three main steps of PCR?

Practice Questions

Q1
What are the three main steps of PCR?
  1. Denaturation, annealing, extension
  2. Denaturation, transcription, translation
  3. Ligation, denaturation, amplification
  4. Amplification, sequencing, visualization

Questions & Step-by-Step Solutions

What are the three main steps of PCR?
  • Step 1: Denaturation - The double-stranded DNA is heated to separate it into two single strands.
  • Step 2: Annealing - The temperature is lowered to allow short DNA primers to attach to the single strands.
  • Step 3: Extension - The temperature is raised again so that a special enzyme can add new DNA nucleotides to the primers, creating new strands of DNA.
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