In immunohistochemistry, what is the role of the primary antibody?
Practice Questions
Q1
In immunohistochemistry, what is the role of the primary antibody?
To bind to the target antigen
To amplify the signal
To visualize the tissue
To fix the tissue sample
Questions & Step-by-Step Solutions
In immunohistochemistry, what is the role of the primary antibody?
Step 1: Understand that immunohistochemistry is a technique used to visualize specific proteins in tissue samples.
Step 2: Identify that the primary antibody is a special protein designed to recognize and bind to a specific target antigen (the protein of interest) in the tissue.
Step 3: Know that when the primary antibody binds to the target antigen, it forms a complex that can be detected and visualized.
Step 4: Realize that this binding is crucial because it allows researchers to see where the target protein is located in the tissue sample.
