Which immunological technique is used to separate proteins based on their size?

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Question: Which immunological technique is used to separate proteins based on their size?

Options:

Correct Answer: Western blotting

Solution:

Western blotting is used to separate proteins based on their size and then detect specific proteins using antibodies.

Which immunological technique is used to separate proteins based on their size?

Which immunological technique is used to separate proteins based on their size?

Step 1: Understand that proteins are molecules made up of amino acids and can vary in size.
Step 2: Learn that scientists often need to separate these proteins to study them.
Step 3: Know that one common method to separate proteins is called Western blotting.
Step 4: In Western blotting, proteins are first placed in a gel and an electric current is applied.
Step 5: The electric current causes the proteins to move through the gel, with smaller proteins moving faster than larger ones.
Step 6: After separation, the proteins are transferred to a membrane.
Step 7: Specific antibodies are then used to detect the proteins of interest on the membrane.

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