What is the role of the stationary phase in HPLC?

Practice Questions

Q1
What is the role of the stationary phase in HPLC?
  1. To provide a mobile phase
  2. To separate analytes based on their interactions
  3. To inject the sample
  4. To detect the analytes

Questions & Step-by-Step Solutions

What is the role of the stationary phase in HPLC?
  • Step 1: Understand that HPLC stands for High-Performance Liquid Chromatography, a technique used to separate different substances in a mixture.
  • Step 2: Identify the stationary phase, which is a solid or a liquid that is fixed in place inside the HPLC column.
  • Step 3: Recognize that the analytes (the substances being separated) pass through the column and interact with the stationary phase.
  • Step 4: Learn that different analytes interact with the stationary phase in different ways, which affects how quickly they move through the column.
  • Step 5: Conclude that the role of the stationary phase is to help separate the analytes based on these interactions, allowing for their identification and quantification.
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