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What is the purpose of a DNA ladder in gel electrophoresis?

Practice Questions

Q1
What is the purpose of a DNA ladder in gel electrophoresis?
  1. To visualize the gel
  2. To provide a size reference for DNA fragments
  3. To enhance the resolution of bands
  4. To stain the DNA

Questions & Step-by-Step Solutions

What is the purpose of a DNA ladder in gel electrophoresis?
  • Step 1: Understand that gel electrophoresis is a technique used to separate DNA fragments based on their size.
  • Step 2: Know that a DNA ladder is a mixture of DNA fragments of known sizes.
  • Step 3: When you run the gel electrophoresis, you also load the DNA ladder into one of the wells of the gel.
  • Step 4: After the gel is run, the DNA ladder will create bands at specific positions that correspond to its known sizes.
  • Step 5: You can then compare the positions of your DNA samples' bands to the bands of the DNA ladder.
  • Step 6: This comparison helps you determine the sizes of your DNA fragments.
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