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What is the role of the buffer in gel electrophoresis?
Practice Questions
Q1
What is the role of the buffer in gel electrophoresis?
To provide nutrients to the gel
To maintain pH and conduct electricity
To stain the DNA
To solidify the gel
Questions & Step-by-Step Solutions
What is the role of the buffer in gel electrophoresis?
Steps
Concepts
Step 1: Understand that gel electrophoresis is a technique used to separate DNA or proteins.
Step 2: Know that a gel is used to hold the samples and allow them to move.
Step 3: Learn that a buffer solution is added to the gel and the electrophoresis apparatus.
Step 4: Recognize that the buffer helps maintain a stable pH, which is important for the samples to stay intact.
Step 5: Understand that the buffer conducts electricity, which is necessary for the DNA or proteins to move through the gel.
Step 6: Conclude that without the buffer, the samples would not migrate properly, and the results would be inaccurate.
No concepts available.
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