What type of chromatography is commonly used for separating proteins?
Practice Questions
Q1
What type of chromatography is commonly used for separating proteins?
Gas chromatography
Affinity chromatography
Paper chromatography
Column chromatography
Questions & Step-by-Step Solutions
What type of chromatography is commonly used for separating proteins?
Step 1: Understand what chromatography is. It is a technique used to separate different substances in a mixture.
Step 2: Learn about proteins. Proteins are large molecules made up of amino acids and have specific functions in the body.
Step 3: Know that there are different types of chromatography, but some are better for proteins than others.
Step 4: Identify affinity chromatography. This type of chromatography separates proteins based on their specific interactions with other molecules.
Step 5: Realize that in affinity chromatography, a protein will bind to a specific ligand (a molecule it interacts with) on a column, allowing other substances to wash away.
Step 6: Finally, understand that after washing, the bound protein can be eluted (washed off) from the column, allowing for its separation and collection.