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What does Km represent in enzyme kinetics?

Practice Questions

Q1
What does Km represent in enzyme kinetics?
  1. The maximum velocity of the reaction
  2. The substrate concentration at which the reaction rate is half of Vmax
  3. The rate constant of the reaction
  4. The enzyme's affinity for the substrate

Questions & Step-by-Step Solutions

What does Km represent in enzyme kinetics?
  • Step 1: Understand that Km is a value used in enzyme kinetics.
  • Step 2: Know that Km stands for Michaelis constant.
  • Step 3: Recognize that Km is related to the concentration of substrate (the molecule the enzyme acts on).
  • Step 4: Learn that Km is the substrate concentration at which the reaction rate is half of the maximum rate (Vmax).
  • Step 5: Understand that a lower Km value means the enzyme has a higher affinity for the substrate, while a higher Km means lower affinity.
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