Which downstream processing step is typically performed last?

Practice Questions

Q1
Which downstream processing step is typically performed last?
  1. Filtration
  2. Chromatography
  3. Formulation
  4. Lyophilization

Questions & Step-by-Step Solutions

Which downstream processing step is typically performed last?
  • Step 1: Understand what downstream processing means. It refers to the steps taken after the initial production of a product, like a drug or protein, to purify and prepare it for use.
  • Step 2: Identify the common steps in downstream processing. These can include filtration, chromatography, and concentration.
  • Step 3: Learn about lyophilization. It is a process that removes water from a product by freezing it and then reducing the pressure to allow the ice to sublimate.
  • Step 4: Recognize that lyophilization is used to stabilize products for long-term storage by preventing degradation.
  • Step 5: Conclude that since lyophilization is the final step that ensures the product is stable and ready for storage, it is typically performed last in downstream processing.
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