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What is the purpose of buffer exchange in downstream processing?

Practice Questions

Q1
What is the purpose of buffer exchange in downstream processing?
  1. To increase product yield
  2. To remove contaminants
  3. To adjust pH and ionic strength
  4. To enhance solubility

Questions & Step-by-Step Solutions

What is the purpose of buffer exchange in downstream processing?
  • Step 1: Understand that buffer exchange is a process used in biochemistry.
  • Step 2: Know that buffers help maintain a stable pH level in solutions.
  • Step 3: Realize that different purification steps require specific pH and ionic strength.
  • Step 4: Learn that buffer exchange replaces the current buffer with a new one that has the desired pH and ionic strength.
  • Step 5: Recognize that this adjustment helps improve the efficiency of the purification process.
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