What is the role of chromatography in downstream processing?
Practice Questions
Q1
What is the role of chromatography in downstream processing?
To kill microorganisms
To concentrate the product
To separate and purify biomolecules
To enhance product stability
Questions & Step-by-Step Solutions
What is the role of chromatography in downstream processing?
Step 1: Understand that chromatography is a technique used in laboratories.
Step 2: Know that it helps to separate different substances from a mixture.
Step 3: Realize that in downstream processing, we want to isolate specific biomolecules (like proteins or DNA) from a complex mixture.
Step 4: Learn that chromatography works by using a stationary phase (a solid or liquid) and a mobile phase (a liquid or gas) to move the mixture through.
Step 5: Recognize that different biomolecules will move at different speeds based on their properties (like size, charge, or affinity to the stationary phase).
Step 6: Conclude that this separation allows scientists to collect and purify the desired biomolecules for further use.
