What is the significance of the 'Golden Gate' cloning method? (2023)
Practice Questions
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What is the significance of the 'Golden Gate' cloning method? (2023)
It allows for the cloning of large DNA fragments
It uses a single restriction enzyme for multiple insertions
It is a method for sequencing DNA
It enhances the stability of plasmids
The 'Golden Gate' cloning method allows for the simultaneous insertion of multiple DNA fragments using a single restriction enzyme, making it efficient for constructing complex plasmids.
Questions & Step-by-step Solutions
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Q
Q: What is the significance of the 'Golden Gate' cloning method? (2023)
Solution: The 'Golden Gate' cloning method allows for the simultaneous insertion of multiple DNA fragments using a single restriction enzyme, making it efficient for constructing complex plasmids.