What is the significance of the 'sticky ends' produced by restriction enzymes? (
Practice Questions
Q1
What is the significance of the 'sticky ends' produced by restriction enzymes? (2023)
They prevent DNA degradation
They facilitate the ligation of DNA fragments
They enhance transcription
They stabilize the DNA structure
Questions & Step-by-Step Solutions
What is the significance of the 'sticky ends' produced by restriction enzymes? (2023)
Step 1: Understand what restriction enzymes are. They are proteins that cut DNA at specific sequences.
Step 2: Learn what 'sticky ends' are. When restriction enzymes cut DNA, they can leave overhanging ends, which are called sticky ends.
Step 3: Know why sticky ends are important. These overhanging ends can easily pair with complementary sequences of DNA.
Step 4: Realize how this helps in DNA manipulation. When you want to join two pieces of DNA together, sticky ends make it easier because they can stick to each other.
Step 5: Understand the process of ligation. After the sticky ends pair up, an enzyme called ligase can join them permanently, creating a new DNA molecule.
