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What is the purpose of using a selectable marker in a cloning vector? (2021)

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Question: What is the purpose of using a selectable marker in a cloning vector? (2021)

Options:

  1. To enhance the stability of the vector
  2. To allow for the identification of successfully transformed cells
  3. To increase the replication rate of the vector
  4. To facilitate the insertion of foreign DNA

Correct Answer: To allow for the identification of successfully transformed cells

Exam Year: 2021

Solution: 

Selectable markers are genes that confer resistance to antibiotics or other substances, allowing for the identification of cells that have successfully taken up the vector.

What is the purpose of using a selectable marker in a cloning vector? (2021)

Practice Questions

Q1
What is the purpose of using a selectable marker in a cloning vector? (2021)
  1. To enhance the stability of the vector
  2. To allow for the identification of successfully transformed cells
  3. To increase the replication rate of the vector
  4. To facilitate the insertion of foreign DNA

Questions & Step-by-Step Solutions

What is the purpose of using a selectable marker in a cloning vector? (2021)
  • Step 1: Understand what a cloning vector is. A cloning vector is a tool used to insert a piece of DNA into a host cell.
  • Step 2: Learn about selectable markers. Selectable markers are special genes included in the cloning vector.
  • Step 3: Know the function of selectable markers. They help identify which cells have successfully taken up the cloning vector.
  • Step 4: Recognize how they work. Selectable markers often provide resistance to antibiotics or other harmful substances.
  • Step 5: Identify successful cells. When you apply the antibiotic, only the cells that have the cloning vector (with the selectable marker) will survive.
  • Step 6: Conclude the purpose. This allows researchers to easily find and select the cells that have the desired DNA inserted.
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