What is the purpose of using a selectable marker in a cloning vector? (2021)

What is the purpose of using a selectable marker in a cloning vector? (2021)

Step 1: Understand what a cloning vector is. A cloning vector is a tool used to insert a piece of DNA into a host cell.
Step 2: Learn about selectable markers. Selectable markers are special genes included in the cloning vector.
Step 3: Know the function of selectable markers. They help identify which cells have successfully taken up the cloning vector.
Step 4: Recognize how they work. Selectable markers often provide resistance to antibiotics or other harmful substances.
Step 5: Identify successful cells. When you apply the antibiotic, only the cells that have the cloning vector (with the selectable marker) will survive.
Step 6: Conclude the purpose. This allows researchers to easily find and select the cells that have the desired DNA inserted.

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