What is the function of the selectable marker gene in a recombinant DNA experime

Practice Questions

Q1
What is the function of the selectable marker gene in a recombinant DNA experiment? (2021)
  1. To enhance gene expression
  2. To allow for the identification of successfully transformed cells
  3. To provide energy to the cells
  4. To degrade unwanted DNA

Questions & Step-by-Step Solutions

What is the function of the selectable marker gene in a recombinant DNA experiment? (2021)
  • Step 1: In a recombinant DNA experiment, scientists insert a piece of DNA into a host cell.
  • Step 2: To find out which cells have taken up the new DNA, they use a selectable marker gene.
  • Step 3: The selectable marker gene usually provides a trait, like antibiotic resistance or the ability to grow in specific conditions.
  • Step 4: After introducing the recombinant DNA, the scientists apply a condition (like an antibiotic) that only the cells with the selectable marker can survive.
  • Step 5: The cells that survive are the ones that successfully took up the recombinant DNA, allowing researchers to select and study them.
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