What is the function of the selectable marker gene in a recombinant DNA experime
Practice Questions
Q1
What is the function of the selectable marker gene in a recombinant DNA experiment? (2021)
To enhance gene expression
To allow for the identification of successfully transformed cells
To provide energy to the cells
To degrade unwanted DNA
Questions & Step-by-Step Solutions
What is the function of the selectable marker gene in a recombinant DNA experiment? (2021)
Step 1: In a recombinant DNA experiment, scientists insert a piece of DNA into a host cell.
Step 2: To find out which cells have taken up the new DNA, they use a selectable marker gene.
Step 3: The selectable marker gene usually provides a trait, like antibiotic resistance or the ability to grow in specific conditions.
Step 4: After introducing the recombinant DNA, the scientists apply a condition (like an antibiotic) that only the cells with the selectable marker can survive.
Step 5: The cells that survive are the ones that successfully took up the recombinant DNA, allowing researchers to select and study them.