What is the function of a selectable marker in a cloning vector? (2023)
Practice Questions
1 question
Q1
What is the function of a selectable marker in a cloning vector? (2023)
To enhance gene expression
To allow for the identification of successfully transformed cells
To provide energy for the cell
To stabilize the DNA
Selectable markers are genes that confer resistance to antibiotics or other substances, allowing for the identification of cells that have successfully taken up the vector.
Questions & Step-by-step Solutions
1 item
Q
Q: What is the function of a selectable marker in a cloning vector? (2023)
Solution: Selectable markers are genes that confer resistance to antibiotics or other substances, allowing for the identification of cells that have successfully taken up the vector.
Steps: 6
Step 1: Understand what a cloning vector is. A cloning vector is a tool used in genetic engineering to insert and replicate DNA in a host cell.
Step 2: Learn about selectable markers. Selectable markers are specific genes included in the cloning vector.
Step 3: Know the purpose of selectable markers. They help identify which cells have successfully taken up the cloning vector.
Step 4: Recognize how they work. Selectable markers often provide resistance to antibiotics or other harmful substances.
Step 5: Identify successful cells. When the host cells are exposed to the antibiotic, only those that have the selectable marker will survive.
Step 6: Conclude that selectable markers are essential for selecting and identifying cells that contain the desired DNA.
