What is the function of a selectable marker in a cloning vector? (2023)

What is the function of a selectable marker in a cloning vector? (2023)

Step 1: Understand what a cloning vector is. A cloning vector is a tool used in genetic engineering to insert and replicate DNA in a host cell.
Step 2: Learn about selectable markers. Selectable markers are specific genes included in the cloning vector.
Step 3: Know the purpose of selectable markers. They help identify which cells have successfully taken up the cloning vector.
Step 4: Recognize how they work. Selectable markers often provide resistance to antibiotics or other harmful substances.
Step 5: Identify successful cells. When the host cells are exposed to the antibiotic, only those that have the selectable marker will survive.
Step 6: Conclude that selectable markers are essential for selecting and identifying cells that contain the desired DNA.

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