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What is the role of a buffer solution in electrophoresis?
Practice Questions
Q1
What is the role of a buffer solution in electrophoresis?
To enhance the conductivity of the gel
To maintain a constant pH during the separation
To increase the size of the molecules
To provide a source of ions
Questions & Step-by-Step Solutions
What is the role of a buffer solution in electrophoresis?
Steps
Concepts
Step 1: Understand that electrophoresis is a technique used to separate charged molecules, like DNA or proteins, based on their size and charge.
Step 2: Know that a buffer solution is a special liquid that helps keep the pH level stable during the electrophoresis process.
Step 3: Realize that maintaining a constant pH is important because it ensures that the charged molecules behave predictably and separate correctly.
Step 4: Remember that if the pH changes, it can affect the charge of the molecules, leading to poor separation or inaccurate results.
No concepts available.
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