What is the principle behind the use of chromatography for qualitative analysis?
Practice Questions
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What is the principle behind the use of chromatography for qualitative analysis?
Separation based on charge
Separation based on size
Separation based on solubility
Separation based on boiling point
Chromatography separates components based on their solubility in the mobile phase relative to the stationary phase.
Questions & Step-by-step Solutions
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Q
Q: What is the principle behind the use of chromatography for qualitative analysis?
Solution: Chromatography separates components based on their solubility in the mobile phase relative to the stationary phase.
Steps: 5
Step 1: Understand that chromatography is a technique used to separate different substances in a mixture.
Step 2: Identify the two main phases in chromatography: the stationary phase (the material that stays still) and the mobile phase (the liquid or gas that moves).
Step 3: Recognize that different substances in the mixture have different levels of solubility in the mobile phase.
Step 4: Realize that when the mobile phase moves through the stationary phase, substances that are more soluble will travel further than those that are less soluble.
Step 5: Conclude that this difference in movement allows us to separate and identify the components of the mixture based on how far they travel.