Which technique is best suited for the separation of charged biomolecules?

Practice Questions

Q1
Which technique is best suited for the separation of charged biomolecules?
  1. Gas chromatography
  2. High-performance liquid chromatography
  3. Capillary electrophoresis
  4. Thin-layer chromatography

Questions & Step-by-Step Solutions

Which technique is best suited for the separation of charged biomolecules?
  • Step 1: Understand that biomolecules can have a charge, which means they can be positively or negatively charged.
  • Step 2: Learn that separating charged biomolecules is important for studying their properties and functions.
  • Step 3: Know that there are different techniques to separate biomolecules, but we need one that works well for charged ones.
  • Step 4: Discover that capillary electrophoresis is a technique that uses an electric field to move charged molecules through a gel or liquid.
  • Step 5: Realize that in capillary electrophoresis, molecules are separated based on their charge-to-mass ratio, meaning how much charge they have compared to their size.
  • Step 6: Conclude that because of this ability to separate based on charge, capillary electrophoresis is the best technique for charged biomolecules.
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