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What is the principle behind electrophoresis in qualitative analysis?
What is the principle behind electrophoresis in qualitative analysis?
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What is the principle behind electrophoresis in qualitative analysis?
Separation based on boiling point
Separation based on charge and size
Separation based on solubility
Separation based on density
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Electrophoresis separates charged particles based on their size and charge when an electric field is applied.
Questions & Step-by-step Solutions
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Q: What is the principle behind electrophoresis in qualitative analysis?
Solution:
Electrophoresis separates charged particles based on their size and charge when an electric field is applied.
Steps: 6
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Step 1: Understand that electrophoresis is a method used to separate particles.
Step 2: Know that the particles being separated are usually charged, like proteins or DNA.
Step 3: Realize that an electric field is created, which causes the charged particles to move.
Step 4: Remember that the movement of particles depends on two things: their size and their charge.
Step 5: Smaller or more highly charged particles will move faster through the medium (like a gel).
Step 6: As the particles move, they separate from each other based on their size and charge.
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