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What is the principle behind electrophoresis in qualitative analysis?

Practice Questions

Q1
What is the principle behind electrophoresis in qualitative analysis?
  1. Separation based on boiling point
  2. Separation based on charge and size
  3. Separation based on solubility
  4. Separation based on density

Questions & Step-by-Step Solutions

What is the principle behind electrophoresis in qualitative analysis?
  • Step 1: Understand that electrophoresis is a method used to separate particles.
  • Step 2: Know that the particles being separated are usually charged, like proteins or DNA.
  • Step 3: Realize that an electric field is created, which causes the charged particles to move.
  • Step 4: Remember that the movement of particles depends on two things: their size and their charge.
  • Step 5: Smaller or more highly charged particles will move faster through the medium (like a gel).
  • Step 6: As the particles move, they separate from each other based on their size and charge.
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