In UV-Vis spectroscopy, what does a peak at 280 nm typically indicate?

In UV-Vis spectroscopy, what does a peak at 280 nm typically indicate?

Step 1: Understand what UV-Vis spectroscopy is. It is a technique used to measure how much light is absorbed by a sample at different wavelengths.
Step 2: Know that different substances absorb light at specific wavelengths. This can help identify what is in the sample.
Step 3: Recognize that proteins are made up of building blocks called amino acids.
Step 4: Learn that some amino acids, like tryptophan and tyrosine, have special structures called aromatic rings.
Step 5: Understand that these aromatic amino acids absorb light strongly at a wavelength of 280 nm.
Step 6: Conclude that if you see a peak at 280 nm in a UV-Vis spectrum, it usually means that proteins are present in the sample.

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