What is the purpose of using a blank sample in UV-Vis spectroscopy?

Practice Questions

Q1
What is the purpose of using a blank sample in UV-Vis spectroscopy?
  1. To calibrate the instrument
  2. To increase the sensitivity of the measurement
  3. To provide a reference for baseline correction
  4. To enhance the sample's absorbance

Questions & Step-by-Step Solutions

What is the purpose of using a blank sample in UV-Vis spectroscopy?
  • Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
  • Step 2: Know that a blank sample is a solution that contains everything except the analyte (the substance you want to measure).
  • Step 3: Use the blank sample to measure the absorbance of the solvent and any other components in the solution.
  • Step 4: Subtract the absorbance of the blank from the absorbance of the sample with the analyte.
  • Step 5: This subtraction gives you the true absorbance due only to the analyte, allowing for accurate measurements.
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