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What is the purpose of using a blank sample in UV-Vis spectroscopy?
Practice Questions
Q1
What is the purpose of using a blank sample in UV-Vis spectroscopy?
To calibrate the instrument
To increase the sensitivity of the measurement
To provide a reference for baseline correction
To enhance the sample's absorbance
Questions & Step-by-Step Solutions
What is the purpose of using a blank sample in UV-Vis spectroscopy?
Steps
Concepts
Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
Step 2: Know that a blank sample is a solution that contains everything except the analyte (the substance you want to measure).
Step 3: Use the blank sample to measure the absorbance of the solvent and any other components in the solution.
Step 4: Subtract the absorbance of the blank from the absorbance of the sample with the analyte.
Step 5: This subtraction gives you the true absorbance due only to the analyte, allowing for accurate measurements.
No concepts available.
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