Intro to Spectroscopy (UV-Vis, IR) - Applications

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The Intro to Spectroscopy (UV–Vis, IR) – Applications section highlights the practical and real-world uses of spectroscopic techniques in chemical analysis, research, industry, and quality control. This module is designed for Class 11–12 students and undergraduate learners, helping them connect spectroscopic theory with real-life chemical applications.

In this section, you will explore:

  • Applications of UV–Visible spectroscopy – concentration determination, reaction kinetics monitoring, and colorimetric analysis

  • Qualitative identification of compounds using characteristic UV–Vis absorption bands

  • Infrared (IR) spectroscopy applications – functional group identification and structural confirmation

  • Organic compound analysis – detection of alcohols, acids, carbonyls, and aromatic compounds

  • Industrial and pharmaceutical uses – purity testing, drug analysis, and quality assurance

  • Environmental applications – pollutant detection and water analysis

  • Biochemical and biological relevance – analysis of proteins, nucleic acids, and biomolecules (introductory)

  • Forensic and material analysis – identification of unknown samples and polymers

  • NCERT- and UG-aligned explanations, supported by real spectra examples, case-based questions, MCQs, and application-oriented numericals

The content is structured to enhance application-based reasoning, develop interpretation skills, and prepare students for practical exams, viva questions, and case-based assessments.

Develop a practical understanding of UV–Vis and IR spectroscopy by exploring their applications across science and industry, strengthening both academic performance and analytical insight.

Q. In UV-Vis spectroscopy, what does the Beer-Lambert law relate?
  • A. Wavelength and energy
  • B. Absorbance and concentration
  • C. Frequency and intensity
  • D. Temperature and pressure
Q. What does the peak position in an IR spectrum indicate?
  • A. Concentration of the sample
  • B. Molecular weight of the compound
  • C. Type of functional groups present
  • D. Purity of the sample
Q. What is a common use of UV-Vis spectroscopy in environmental analysis?
  • A. Measuring soil pH
  • B. Detecting heavy metals
  • C. Analyzing water quality
  • D. Identifying microorganisms
Q. What is the main advantage of using FTIR over traditional IR spectroscopy?
  • A. Higher resolution
  • B. Faster data acquisition
  • C. Lower cost
  • D. Simpler sample preparation
Q. What is the primary application of UV-Vis spectroscopy in analytical chemistry?
  • A. Determining molecular weight
  • B. Identifying functional groups
  • C. Quantifying concentrations of analytes
  • D. Separating mixtures
Q. What type of information can be obtained from a UV-Vis spectrum?
  • A. Molecular structure
  • B. Concentration of ions
  • C. Presence of functional groups
  • D. All of the above
Q. What type of information can be obtained from the IR spectrum of a compound?
  • A. Molecular weight
  • B. Functional groups present
  • C. Concentration of the compound
  • D. Thermal stability
Q. Which of the following compounds would you expect to absorb UV light?
  • A. Alkanes
  • B. Aromatic compounds
  • C. Alcohols
  • D. Carboxylic acids
Q. Which of the following is a common application of UV-Vis spectroscopy in the pharmaceutical industry?
  • A. Determining pH of solutions
  • B. Analyzing drug stability
  • C. Measuring melting points
  • D. Identifying impurities
Q. Which of the following is NOT a typical application of IR spectroscopy?
  • A. Identifying organic compounds
  • B. Measuring the concentration of solutions
  • C. Studying molecular vibrations
  • D. Analyzing gas samples
Q. Which type of sample is most suitable for analysis by IR spectroscopy?
  • A. Gaseous samples
  • B. Solid samples only
  • C. Liquid samples only
  • D. All of the above
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