Q. What is the main advantage of using affinity chromatography?
-
A.
Cost-effectiveness
-
B.
High selectivity
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C.
Simplicity of operation
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D.
Speed of processing
Solution
Affinity chromatography offers high selectivity for the target biomolecule, allowing for effective purification.
Correct Answer:
B
— High selectivity
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Q. What is the primary goal of downstream processing in biopharmaceutical production?
-
A.
To enhance cell growth
-
B.
To purify the product
-
C.
To increase fermentation yield
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D.
To reduce production costs
Solution
The primary goal of downstream processing is to purify the product from the fermentation broth.
Correct Answer:
B
— To purify the product
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Q. What is the purpose of buffer exchange in downstream processing?
-
A.
To increase product yield
-
B.
To remove contaminants
-
C.
To adjust pH and ionic strength
-
D.
To enhance solubility
Solution
Buffer exchange is performed to adjust pH and ionic strength for optimal conditions in subsequent purification steps.
Correct Answer:
C
— To adjust pH and ionic strength
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Q. What is the purpose of lyophilization in downstream processing?
-
A.
To purify proteins
-
B.
To remove water from the product
-
C.
To enhance product activity
-
D.
To increase product solubility
Solution
Lyophilization is used to remove water from the product, stabilizing it for long-term storage.
Correct Answer:
B
— To remove water from the product
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Q. What is the role of chromatography in downstream processing?
-
A.
To kill microorganisms
-
B.
To concentrate the product
-
C.
To separate and purify biomolecules
-
D.
To enhance product stability
Solution
Chromatography is used to separate and purify biomolecules based on their properties.
Correct Answer:
C
— To separate and purify biomolecules
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Q. Which downstream processing step is typically performed last?
-
A.
Filtration
-
B.
Chromatography
-
C.
Formulation
-
D.
Lyophilization
Solution
Lyophilization is typically performed last to stabilize the final product for storage.
Correct Answer:
D
— Lyophilization
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Q. Which of the following is a common challenge in downstream processing?
-
A.
High product yield
-
B.
Contamination
-
C.
Low operational costs
-
D.
Rapid processing times
Solution
Contamination is a common challenge in downstream processing that can affect product quality.
Correct Answer:
B
— Contamination
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Q. Which of the following is a common method for protein concentration?
-
A.
Dialysis
-
B.
Ultrafiltration
-
C.
Lyophilization
-
D.
Precipitation
Solution
Ultrafiltration is a common method used for concentrating proteins in downstream processing.
Correct Answer:
B
— Ultrafiltration
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Q. Which technique is commonly used for the clarification of fermentation broth?
-
A.
Chromatography
-
B.
Filtration
-
C.
Centrifugation
-
D.
Precipitation
Solution
Centrifugation is commonly used to separate cells and debris from the fermentation broth.
Correct Answer:
C
— Centrifugation
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