Immobilized enzymes are used in various applications, including biosensors, drug synthesis, and food processing.

Temperature can significantly affect immobilized enzyme activity, potentially leading to denaturation at high temperatures.

An immobilized enzyme is one that is attached to a solid support, which allows for easier separation from the reaction mixture.

One advantage of using immobilized enzymes is easier enzyme recovery, which simplifies the purification process after the reaction.

The primary purpose of immobilizing enzymes is to enhance their stability and reusability in various applications.

The role of a carrier in enzyme immobilization is to provide a surface for enzyme attachment, facilitating the immobilization process.

Covalent bonding is a common method for immobilizing enzymes, allowing them to remain attached to a support while retaining activity.

A disadvantage of immobilized enzymes can be reduced enzyme activity due to steric hindrance or changes in the enzyme's conformation.

While pH, temperature, and support material affect the activity of immobilized enzymes, enzyme concentration is typically controlled in the reaction mixture.

All of the mentioned supports, including cellulose, silica gel, and polyacrylamide, are commonly used for enzyme immobilization.