Q. What is a common challenge associated with using viral vectors in animal cell culture?
A.
Low transfection efficiency
B.
Immune response from the host
C.
High cost of production
D.
Limited insert size
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Solution
A significant challenge with viral vectors is the potential immune response from the host, which can limit their effectiveness.
Correct Answer:
B
— Immune response from the host
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Q. What is a common feature of plasmid vectors used in animal cell culture?
A.
Ability to replicate independently
B.
Inability to carry antibiotic resistance genes
C.
Lack of a promoter region
D.
High molecular weight
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Solution
Plasmid vectors can replicate independently within the host cell, which is essential for gene cloning and expression.
Correct Answer:
A
— Ability to replicate independently
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Q. What is the primary purpose of using vectors in animal cell culture?
A.
To enhance cell growth
B.
To introduce foreign DNA
C.
To provide nutrients
D.
To increase cell size
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Solution
Vectors are primarily used to introduce foreign DNA into animal cells for various applications, including gene expression and protein production.
Correct Answer:
B
— To introduce foreign DNA
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Q. What is the role of a selectable marker in a vector?
A.
To enhance cell adhesion
B.
To allow for the selection of successfully transfected cells
C.
To increase protein expression
D.
To provide energy to the cells
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Solution
Selectable markers are used to identify and select cells that have successfully incorporated the vector.
Correct Answer:
B
— To allow for the selection of successfully transfected cells
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Q. What is the significance of a promoter in a vector?
A.
It provides structural support to the vector
B.
It regulates the expression of the inserted gene
C.
It enhances cell division
D.
It prevents vector degradation
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Solution
A promoter is crucial as it regulates the expression of the inserted gene, determining when and how much of the gene product is made.
Correct Answer:
B
— It regulates the expression of the inserted gene
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Q. Which of the following is NOT a common application of vectors in animal cell culture?
A.
Gene expression studies
B.
Protein production
C.
Cellular metabolism analysis
D.
Gene therapy
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Solution
Cellular metabolism analysis is not a direct application of vectors; vectors are primarily used for gene expression, protein production, and gene therapy.
Correct Answer:
C
— Cellular metabolism analysis
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Q. Which of the following vectors can carry the largest DNA inserts?
A.
Plasmid vectors
B.
Viral vectors
C.
Bacterial artificial chromosomes
D.
Cosmids
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Solution
Bacterial artificial chromosomes (BACs) can carry larger DNA inserts compared to plasmids, viral vectors, or cosmids.
Correct Answer:
C
— Bacterial artificial chromosomes
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Q. Which type of vector is commonly used for stable transfection in animal cells?
A.
Plasmid vectors
B.
Bacteriophage vectors
C.
Viral vectors
D.
Cosmids
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Solution
Viral vectors are often used for stable transfection in animal cells due to their ability to integrate into the host genome.
Correct Answer:
C
— Viral vectors
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Q. Which type of vector is typically used for transient expression in animal cells?
A.
Viral vectors
B.
Plasmid vectors
C.
Bacterial artificial chromosomes
D.
Yeast artificial chromosomes
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Solution
Plasmid vectors are commonly used for transient expression in animal cells, allowing for short-term gene expression.
Correct Answer:
B
— Plasmid vectors
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Q. Which viral vector is most commonly used for gene therapy in animal cells?
A.
Adenovirus
B.
Bacteriophage
C.
Lentivirus
D.
Herpesvirus
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Solution
Lentivirus is commonly used in gene therapy due to its ability to integrate into the host genome and infect non-dividing cells.
Correct Answer:
C
— Lentivirus
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