Q. What is the function of trypsin in cell culture?
-
A.
To promote cell growth
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B.
To detach adherent cells from the culture surface
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C.
To provide nutrients
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D.
To buffer the medium
Solution
Trypsin is an enzyme used to detach adherent cells from the culture surface, allowing for passaging and subculturing.
Correct Answer:
B
— To detach adherent cells from the culture surface
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Q. What is the primary purpose of using animal cell culture in research?
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A.
To study cellular processes in a controlled environment
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B.
To produce large quantities of bacteria
-
C.
To create genetically modified plants
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D.
To synthesize inorganic compounds
Solution
Animal cell culture allows researchers to study cellular processes in a controlled environment, providing insights into biology and disease.
Correct Answer:
A
— To study cellular processes in a controlled environment
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Q. What is the purpose of using antibiotics in cell culture?
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A.
To promote cell growth
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B.
To prevent contamination
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C.
To enhance cell differentiation
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D.
To increase cell adhesion
Solution
Antibiotics are used in cell culture to prevent bacterial contamination, ensuring the integrity of the cell culture.
Correct Answer:
B
— To prevent contamination
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Q. What is the role of serum in animal cell culture?
-
A.
To provide a source of vitamins and hormones
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B.
To inhibit cell growth
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C.
To act as a buffer
-
D.
To provide a solid surface for attachment
Solution
Serum provides essential growth factors, hormones, and nutrients necessary for the growth and maintenance of animal cells in culture.
Correct Answer:
A
— To provide a source of vitamins and hormones
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Q. What is the significance of passaging in cell culture?
-
A.
To increase the number of cells
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B.
To decrease the cell density
-
C.
To change the medium
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D.
To freeze the cells
Solution
Passaging is the process of transferring cells to new culture vessels to increase the number of cells and prevent overcrowding.
Correct Answer:
A
— To increase the number of cells
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Q. What is the typical pH range for animal cell culture media?
-
A.
4.0 - 5.0
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B.
6.5 - 7.5
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C.
8.0 - 9.0
-
D.
10.0 - 11.0
Solution
The typical pH range for animal cell culture media is 6.5 to 7.5, which is optimal for cell growth and function.
Correct Answer:
B
— 6.5 - 7.5
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Q. Which of the following is a common contaminant in animal cell cultures?
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A.
Fungi
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B.
Bacteria
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C.
Mycoplasma
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D.
All of the above
Solution
All of the above are common contaminants in animal cell cultures, which can adversely affect cell growth and experimental results.
Correct Answer:
D
— All of the above
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Q. Which of the following is a common type of medium used for animal cell culture?
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A.
Nutrient agar
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B.
Luria-Bertani broth
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C.
Dulbecco's Modified Eagle Medium (DMEM)
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D.
Potato dextrose agar
Solution
Dulbecco's Modified Eagle Medium (DMEM) is a widely used culture medium for animal cells, providing essential nutrients.
Correct Answer:
C
— Dulbecco's Modified Eagle Medium (DMEM)
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Q. Which of the following is a key factor in maintaining the viability of cultured animal cells?
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A.
Temperature control
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B.
High oxygen levels
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C.
Low humidity
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D.
Excessive nutrient concentration
Solution
Temperature control is crucial for maintaining the viability of cultured animal cells, as they require specific conditions to thrive.
Correct Answer:
A
— Temperature control
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Q. Which of the following is NOT a characteristic of adherent cell cultures?
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A.
Cells grow attached to a surface
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B.
Cells can be easily passaged
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C.
Cells grow in suspension
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D.
Cells require a substrate for attachment
Solution
Adherent cell cultures are characterized by cells growing attached to a surface, while suspension cultures do not require attachment.
Correct Answer:
C
— Cells grow in suspension
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