Q. What is the function of auxins in plant cell culture?
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A.
Promote cell elongation
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B.
Stimulate root formation
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C.
Enhance flowering
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D.
Inhibit growth
Solution
Auxins are plant hormones that primarily promote cell elongation and are crucial for root formation in plant tissue culture.
Correct Answer:
B
— Stimulate root formation
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Q. What is the primary purpose of Murashige and Skoog (MS) medium in plant cell culture?
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A.
To provide a source of vitamins
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B.
To supply essential macro and micronutrients
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C.
To enhance cell division
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D.
To promote root development
Solution
Murashige and Skoog (MS) medium is designed to provide essential macro and micronutrients necessary for plant cell growth and development.
Correct Answer:
B
— To supply essential macro and micronutrients
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Q. What is the purpose of adding vitamins to plant tissue culture media?
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A.
To enhance color
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B.
To provide energy
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C.
To support metabolic functions
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D.
To increase growth rate
Solution
Vitamins are added to support various metabolic functions in plant cells during culture.
Correct Answer:
C
— To support metabolic functions
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Q. What is the significance of pH in plant tissue culture media?
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A.
Affects nutrient availability
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B.
Influences color of the medium
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C.
Determines the solidifying agent
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D.
Regulates temperature
Solution
The pH of plant tissue culture media affects nutrient availability and overall cell growth.
Correct Answer:
A
— Affects nutrient availability
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Q. What role does agar play in plant cell culture media?
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A.
Nutrient source
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B.
pH stabilizer
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C.
Solidifying agent
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D.
Growth regulator
Solution
Agar serves as a solidifying agent in plant cell culture media, providing a stable surface for cell growth.
Correct Answer:
C
— Solidifying agent
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Q. Which component is commonly added to plant tissue culture media to promote shoot regeneration?
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A.
Agar
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B.
Cytokinins
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C.
Sucrose
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D.
Gibberellins
Solution
Cytokinins are plant hormones that promote cell division and are commonly used to enhance shoot regeneration in plant tissue culture.
Correct Answer:
B
— Cytokinins
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Q. Which medium is specifically designed for the culture of orchid tissues?
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A.
Murashige and Skoog
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B.
Gamborg's B5
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C.
Orchid medium
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D.
Nitsch and Nitsch
Solution
Orchid medium is specifically formulated to meet the unique nutritional requirements of orchid tissues.
Correct Answer:
C
— Orchid medium
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Q. Which of the following is NOT a typical component of plant tissue culture media?
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A.
Mineral salts
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B.
Vitamins
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C.
Antibiotics
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D.
Proteins
Solution
Proteins are not typically included in plant tissue culture media, while mineral salts, vitamins, and sometimes antibiotics are used.
Correct Answer:
D
— Proteins
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Q. Which sugar is most commonly used in plant tissue culture media?
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A.
Glucose
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B.
Fructose
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C.
Sucrose
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D.
Mannitol
Solution
Sucrose is the most commonly used sugar in plant tissue culture media as it provides energy for the growing cells.
Correct Answer:
C
— Sucrose
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