Q. What does HPLC stand for?
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A.
High Performance Liquid Chromatography
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B.
High Pressure Liquid Chromatography
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C.
High Precision Liquid Chromatography
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D.
High Purity Liquid Chromatography
Solution
HPLC stands for High Performance Liquid Chromatography, a technique used for separating, identifying, and quantifying components in a mixture.
Correct Answer:
A
— High Performance Liquid Chromatography
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Q. What is the primary purpose of the mobile phase in HPLC?
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A.
To provide a stationary phase
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B.
To dissolve the sample
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C.
To detect the analytes
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D.
To inject the sample
Solution
The primary purpose of the mobile phase in HPLC is to dissolve the sample and carry it through the column.
Correct Answer:
B
— To dissolve the sample
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Q. What is the role of the stationary phase in HPLC?
-
A.
To provide a mobile phase
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B.
To separate analytes based on their interactions
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C.
To inject the sample
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D.
To detect the analytes
Solution
The stationary phase in HPLC separates analytes based on their interactions with the stationary material.
Correct Answer:
B
— To separate analytes based on their interactions
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Q. What is the typical pressure range for HPLC systems?
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A.
0-10 psi
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B.
10-100 psi
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C.
100-5000 psi
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D.
5000-10000 psi
Solution
The typical pressure range for HPLC systems is 100-5000 psi, which is necessary for efficient separation.
Correct Answer:
C
— 100-5000 psi
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Q. What type of chromatography is HPLC classified as?
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A.
Gas chromatography
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B.
Liquid chromatography
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C.
Thin-layer chromatography
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D.
Paper chromatography
Solution
HPLC is classified as liquid chromatography, as it uses a liquid mobile phase to separate components.
Correct Answer:
B
— Liquid chromatography
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Q. Which component is essential for the separation process in HPLC?
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A.
Detector
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B.
Mobile phase
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C.
Sample injector
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D.
Column
Solution
The column is essential for the separation process in HPLC, as it contains the stationary phase where the separation occurs.
Correct Answer:
D
— Column
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Q. Which factor can affect the resolution in HPLC?
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A.
Column length
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B.
Particle size of stationary phase
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C.
Flow rate
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D.
All of the above
Solution
All of the above factors can affect the resolution in HPLC, influencing the separation quality of analytes.
Correct Answer:
D
— All of the above
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Q. Which of the following is a common application of HPLC?
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A.
Drug testing
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B.
Food analysis
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C.
Environmental monitoring
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D.
All of the above
Solution
HPLC is widely used in drug testing, food analysis, and environmental monitoring, making it a versatile analytical technique.
Correct Answer:
D
— All of the above
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Q. Which type of detector is commonly used in HPLC?
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A.
Mass spectrometer
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B.
UV-Vis spectrophotometer
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C.
Fluorescence detector
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D.
All of the above
Solution
All of the above detectors can be used in HPLC, with UV-Vis spectrophotometers being the most common.
Correct Answer:
D
— All of the above
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