Q. In gas chromatography, what is the role of the carrier gas?
-
A.
To dissolve the sample
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B.
To provide a medium for separation
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C.
To react with the sample
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D.
To cool the system
Solution
The carrier gas provides a medium for separation in gas chromatography.
Correct Answer:
B
— To provide a medium for separation
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Q. In thin-layer chromatography (TLC), what is the purpose of the solvent front?
-
A.
To carry the sample up the plate
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B.
To act as a stationary phase
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C.
To visualize the compounds
-
D.
To cool the plate
Solution
The solvent front carries the sample up the plate in thin-layer chromatography.
Correct Answer:
A
— To carry the sample up the plate
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Q. What is the main advantage of high-performance liquid chromatography (HPLC)?
-
A.
It requires larger sample sizes
-
B.
It provides faster separation and higher resolution
-
C.
It is less expensive than other methods
-
D.
It does not require a solvent
Solution
HPLC provides faster separation and higher resolution compared to other chromatography methods.
Correct Answer:
B
— It provides faster separation and higher resolution
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Q. What is the main disadvantage of using paper chromatography?
-
A.
It is time-consuming
-
B.
It has low resolution
-
C.
It requires expensive equipment
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D.
It cannot separate small molecules
Solution
The main disadvantage of paper chromatography is its low resolution compared to other methods.
Correct Answer:
B
— It has low resolution
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Q. What is the primary purpose of chromatography in a clinical laboratory?
-
A.
To separate and analyze complex mixtures
-
B.
To sterilize medical equipment
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C.
To measure blood pressure
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D.
To perform surgical procedures
Solution
Chromatography is primarily used to separate and analyze complex mixtures in clinical laboratories.
Correct Answer:
A
— To separate and analyze complex mixtures
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Q. What type of chromatography is commonly used for separating proteins?
-
A.
Gas chromatography
-
B.
Affinity chromatography
-
C.
Paper chromatography
-
D.
Column chromatography
Solution
Affinity chromatography is commonly used for separating proteins based on specific interactions.
Correct Answer:
B
— Affinity chromatography
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Q. Which component of chromatography is responsible for the separation of compounds?
-
A.
Mobile phase
-
B.
Stationary phase
-
C.
Detector
-
D.
Sample injector
Solution
The stationary phase is responsible for the separation of compounds in chromatography.
Correct Answer:
B
— Stationary phase
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Q. Which factor does NOT affect the separation in chromatography?
-
A.
Temperature
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B.
Flow rate of the mobile phase
-
C.
Nature of the stationary phase
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D.
Color of the sample
Solution
The color of the sample does not affect the separation in chromatography.
Correct Answer:
D
— Color of the sample
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Q. Which type of chromatography uses a solid stationary phase and a liquid mobile phase?
-
A.
Gas chromatography
-
B.
Liquid chromatography
-
C.
Thin-layer chromatography
-
D.
High-performance liquid chromatography
Solution
Liquid chromatography uses a solid stationary phase and a liquid mobile phase for separation.
Correct Answer:
B
— Liquid chromatography
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