Q. What is the effect of increasing the voltage during gel electrophoresis?
-
A.
Increases the resolution of bands
-
B.
Decreases the migration time
-
C.
Increases the size of the gel
-
D.
Decreases the temperature of the gel
Solution
Increasing the voltage decreases the migration time, allowing the samples to move faster through the gel.
Correct Answer:
B
— Decreases the migration time
Learn More →
Q. What is the primary purpose of gel electrophoresis in molecular biology?
-
A.
To amplify DNA sequences
-
B.
To separate nucleic acids or proteins based on size
-
C.
To sequence DNA
-
D.
To clone genes
Solution
Gel electrophoresis is primarily used to separate nucleic acids or proteins based on their size.
Correct Answer:
B
— To separate nucleic acids or proteins based on size
Learn More →
Q. What is the purpose of a DNA ladder in gel electrophoresis?
-
A.
To visualize the gel
-
B.
To provide a size reference for DNA fragments
-
C.
To enhance the resolution of bands
-
D.
To stain the DNA
Solution
A DNA ladder serves as a size reference for comparing the lengths of DNA fragments in the gel.
Correct Answer:
B
— To provide a size reference for DNA fragments
Learn More →
Q. What is the purpose of staining the gel after electrophoresis?
-
A.
To enhance the gel's structural integrity
-
B.
To visualize the separated DNA or proteins
-
C.
To increase the gel's conductivity
-
D.
To prevent degradation of samples
Solution
Staining the gel allows for visualization of the separated DNA or proteins after electrophoresis.
Correct Answer:
B
— To visualize the separated DNA or proteins
Learn More →
Q. What is the role of the buffer in gel electrophoresis?
-
A.
To provide nutrients to the gel
-
B.
To maintain pH and conduct electricity
-
C.
To stain the DNA
-
D.
To solidify the gel
Solution
The buffer maintains pH and conducts electricity, allowing the DNA or proteins to migrate through the gel.
Correct Answer:
B
— To maintain pH and conduct electricity
Learn More →
Q. What is the typical charge of DNA molecules during gel electrophoresis?
-
A.
Positive
-
B.
Negative
-
C.
Neutral
-
D.
Variable
Solution
DNA molecules have a negative charge due to their phosphate backbone, causing them to migrate towards the positive electrode.
Correct Answer:
B
— Negative
Learn More →
Q. Which of the following factors can affect the resolution of bands in gel electrophoresis?
-
A.
Gel concentration
-
B.
Voltage applied
-
C.
Running time
-
D.
All of the above
Solution
All of the above factors can affect the resolution of bands in gel electrophoresis.
Correct Answer:
D
— All of the above
Learn More →
Q. Which of the following is a common stain used for visualizing DNA in gel electrophoresis?
-
A.
Coomassie Brilliant Blue
-
B.
Ethidium Bromide
-
C.
Silver Nitrate
-
D.
Bromophenol Blue
Solution
Ethidium Bromide is a common stain used for visualizing DNA in gel electrophoresis as it intercalates with DNA.
Correct Answer:
B
— Ethidium Bromide
Learn More →
Q. Which type of gel is commonly used for DNA electrophoresis?
-
A.
Agarose gel
-
B.
Polyacrylamide gel
-
C.
Cellulose acetate
-
D.
Starch gel
Solution
Agarose gel is commonly used for DNA electrophoresis due to its suitable pore size for separating DNA fragments.
Correct Answer:
A
— Agarose gel
Learn More →
Showing 1 to 9 of 9 (1 Pages)
