DNA Sequencing, Mutation and Repair

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Q. What is a common consequence of a frameshift mutation?
  • A. No effect on protein function
  • B. A premature stop codon
  • C. A single amino acid change
  • D. Increased DNA replication speed
Q. What is the function of the enzyme ligase in DNA repair?
  • A. To synthesize new DNA strands
  • B. To unwind the DNA double helix
  • C. To join DNA fragments together
  • D. To remove damaged nucleotides
Q. What is the primary function of mismatch repair?
  • A. To repair single-strand breaks
  • B. To correct base-pairing errors
  • C. To remove damaged bases
  • D. To fix double-strand breaks
Q. What is the primary purpose of DNA sequencing?
  • A. To determine the order of nucleotides in DNA
  • B. To repair damaged DNA
  • C. To replicate DNA
  • D. To transcribe DNA into RNA
Q. What is the role of DNA polymerase in DNA repair?
  • A. To unwind the DNA double helix
  • B. To synthesize new DNA strands
  • C. To remove damaged DNA
  • D. To ligate DNA fragments
Q. Which sequencing method uses fluorescently labeled nucleotides?
  • A. Sanger sequencing
  • B. Next-generation sequencing
  • C. Pyrosequencing
  • D. Capillary electrophoresis
Q. Which type of DNA repair mechanism is used to fix thymine dimers caused by UV light?
  • A. Base excision repair
  • B. Nucleotide excision repair
  • C. Mismatch repair
  • D. Homologous recombination
Q. Which type of mutation can result from exposure to ionizing radiation?
  • A. Point mutation
  • B. Translocation
  • C. Inversion
  • D. All of the above
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