Q. In fermentation kinetics, what does the term 'lag phase' refer to?
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A.
Rapid growth phase
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B.
Adaptation period
-
C.
Stationary phase
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D.
Decline phase
Solution
The lag phase is the adaptation period where microorganisms adjust to their environment before active growth begins.
Correct Answer:
B
— Adaptation period
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Q. In which application are immobilized enzymes commonly used?
-
A.
Biosensors
-
B.
Drug synthesis
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C.
Food processing
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D.
All of the above
Solution
Immobilized enzymes are used in various applications, including biosensors, drug synthesis, and food processing.
Correct Answer:
D
— All of the above
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Q. In which condition is turbulent flow most likely to occur?
-
A.
High viscosity
-
B.
Low velocity
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C.
High flow rate
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D.
Small diameter
Solution
Turbulent flow is most likely to occur at high flow rates, where the inertial forces overcome the viscous forces.
Correct Answer:
C
— High flow rate
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Q. In which scenario is agitation most critical?
-
A.
During a routine check-up
-
B.
In emergency resuscitation
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C.
For chronic pain management
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D.
In physical therapy
Solution
Agitation is critical during emergency resuscitation to ensure effective drug delivery and patient stabilization.
Correct Answer:
B
— In emergency resuscitation
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Q. What does Km represent in enzyme kinetics?
-
A.
The maximum velocity of the reaction
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B.
The substrate concentration at which the reaction rate is half of Vmax
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C.
The rate constant of the reaction
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D.
The enzyme's affinity for the substrate
Solution
Km is the substrate concentration at which the reaction rate is half of Vmax, indicating the enzyme's affinity for the substrate.
Correct Answer:
B
— The substrate concentration at which the reaction rate is half of Vmax
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Q. What effect does increasing temperature generally have on enzyme activity?
-
A.
Increases activity until a certain point
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B.
Decreases activity at all temperatures
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C.
Has no effect on activity
-
D.
Increases activity indefinitely
Solution
Increasing temperature generally increases enzyme activity up to an optimal point, after which activity declines due to denaturation.
Correct Answer:
A
— Increases activity until a certain point
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Q. What effect does increasing the diameter of a blood vessel have on blood flow?
-
A.
Decreases flow
-
B.
Increases flow
-
C.
No effect
-
D.
Causes turbulence
Solution
Increasing the diameter of a blood vessel decreases resistance, thereby increasing blood flow according to Poiseuille's law.
Correct Answer:
B
— Increases flow
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Q. What effect does temperature have on immobilized enzyme activity?
-
A.
No effect
-
B.
Increases activity at all temperatures
-
C.
Can denature the enzyme at high temperatures
-
D.
Only affects free enzymes
Solution
Temperature can significantly affect immobilized enzyme activity, potentially leading to denaturation at high temperatures.
Correct Answer:
C
— Can denature the enzyme at high temperatures
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Q. What is a common microorganism used in fermentation processes?
-
A.
Escherichia coli
-
B.
Staphylococcus aureus
-
C.
Clostridium botulinum
-
D.
Bacillus anthracis
Solution
Escherichia coli is commonly used in fermentation processes for protein production and genetic engineering.
Correct Answer:
A
— Escherichia coli
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Q. What is a potential risk of excessive agitation in a patient?
-
A.
Increased medication absorption
-
B.
Decreased risk of infection
-
C.
Tissue damage
-
D.
Improved respiratory function
Solution
Excessive agitation can lead to tissue damage due to mechanical stress on cells.
Correct Answer:
C
— Tissue damage
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Q. What is an enzyme's active site?
-
A.
The part that binds to substrates
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B.
The part that provides energy
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C.
The part that stabilizes the enzyme
-
D.
The part that transports products
Solution
The active site is the region of the enzyme where substrate molecules bind and undergo a chemical reaction.
Correct Answer:
A
— The part that binds to substrates
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Q. What is an immobilized enzyme?
-
A.
An enzyme that is free in solution
-
B.
An enzyme that is attached to a solid support
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C.
An enzyme that is inactive
-
D.
An enzyme that is produced in large quantities
Solution
An immobilized enzyme is one that is attached to a solid support, which allows for easier separation from the reaction mixture.
Correct Answer:
B
— An enzyme that is attached to a solid support
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Q. What is enzyme inhibition?
-
A.
The process of activating an enzyme
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B.
The process of increasing substrate concentration
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C.
The process of decreasing enzyme activity
-
D.
The process of stabilizing enzyme structure
Solution
Enzyme inhibition refers to the process that decreases enzyme activity, which can occur through various mechanisms.
Correct Answer:
C
— The process of decreasing enzyme activity
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Q. What is one advantage of using immobilized enzymes in industrial processes?
-
A.
Higher enzyme activity
-
B.
Easier enzyme recovery
-
C.
Lower production costs
-
D.
Increased substrate specificity
Solution
One advantage of using immobilized enzymes is easier enzyme recovery, which simplifies the purification process after the reaction.
Correct Answer:
B
— Easier enzyme recovery
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Q. What is reverse osmosis primarily used for in water treatment?
-
A.
Disinfection
-
B.
Desalination
-
C.
Fluoridation
-
D.
pH adjustment
Solution
Reverse osmosis is primarily used for desalination, removing salts and impurities from water.
Correct Answer:
B
— Desalination
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Q. What is the effect of agitation on blood samples during laboratory testing?
-
A.
It has no effect
-
B.
It can cause hemolysis
-
C.
It improves sample quality
-
D.
It decreases sample volume
Solution
Agitation can cause hemolysis, which can interfere with laboratory test results.
Correct Answer:
B
— It can cause hemolysis
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Q. What is the effect of gravity on fluid flow in the human body?
-
A.
Increases flow in all positions
-
B.
Decreases flow in all positions
-
C.
Varies with body position
-
D.
Has no effect
Solution
Gravity affects fluid flow in the body, varying with body position; for example, it increases venous return when standing.
Correct Answer:
C
— Varies with body position
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Q. What is the effect of increasing substrate concentration on fermentation rate?
-
A.
Increases indefinitely
-
B.
Decreases
-
C.
Increases to a point then levels off
-
D.
Has no effect
Solution
Increasing substrate concentration typically increases fermentation rate to a saturation point, after which it levels off.
Correct Answer:
C
— Increases to a point then levels off
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Q. What is the effect of pH on enzyme activity?
-
A.
Enzymes work best at any pH
-
B.
Each enzyme has an optimal pH range
-
C.
pH has no effect on enzyme activity
-
D.
All enzymes are active at neutral pH only
Solution
Each enzyme has an optimal pH range where it functions best; deviations can lead to decreased activity or denaturation.
Correct Answer:
B
— Each enzyme has an optimal pH range
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Q. What is the effect of temperature on enzyme activity?
-
A.
Increases indefinitely
-
B.
Decreases indefinitely
-
C.
Increases to an optimal point then decreases
-
D.
Has no effect
Solution
Enzyme activity typically increases with temperature to an optimal point, after which it decreases due to denaturation.
Correct Answer:
C
— Increases to an optimal point then decreases
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Q. What is the main advantage of using a fed-batch fermentation process?
-
A.
Higher yield
-
B.
Lower cost
-
C.
Faster fermentation
-
D.
Easier to control pH
Solution
The main advantage of fed-batch fermentation is the potential for higher yield due to controlled nutrient addition.
Correct Answer:
A
— Higher yield
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Q. What is the main advantage of using affinity chromatography?
-
A.
Cost-effectiveness
-
B.
High selectivity
-
C.
Simplicity of operation
-
D.
Speed of processing
Solution
Affinity chromatography offers high selectivity for the target biomolecule, allowing for effective purification.
Correct Answer:
B
— High selectivity
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Q. What is the main advantage of using recombinant DNA technology in biochemical engineering?
-
A.
Increased production of natural products
-
B.
Ability to produce proteins with modified properties
-
C.
Reduction of production costs
-
D.
Simplification of fermentation processes
Solution
Recombinant DNA technology allows for the production of proteins with modified properties, enhancing their functionality and application.
Correct Answer:
B
— Ability to produce proteins with modified properties
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Q. What is the main disadvantage of using chemical sterilants?
-
A.
They are ineffective
-
B.
They can be toxic
-
C.
They are time-consuming
-
D.
They are expensive
Solution
The main disadvantage of using chemical sterilants is that they can be toxic to humans and require careful handling.
Correct Answer:
B
— They can be toxic
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Q. What is the main goal of water treatment?
-
A.
To make water taste better
-
B.
To ensure water is safe for consumption
-
C.
To increase water supply
-
D.
To reduce water temperature
Solution
The main goal of water treatment is to ensure that water is safe for human consumption.
Correct Answer:
B
— To ensure water is safe for consumption
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Q. What is the main product of lactic acid fermentation?
-
A.
Ethanol
-
B.
Carbon dioxide
-
C.
Lactic acid
-
D.
Acetic acid
Solution
Lactic acid is the main product of lactic acid fermentation, which occurs in certain bacteria and muscle cells.
Correct Answer:
C
— Lactic acid
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Q. What is the minimum temperature and time required for effective steam sterilization in an autoclave?
-
A.
121°C for 15 minutes
-
B.
100°C for 30 minutes
-
C.
134°C for 10 minutes
-
D.
150°C for 5 minutes
Solution
The minimum temperature and time for effective steam sterilization in an autoclave is 121°C for 15 minutes.
Correct Answer:
A
— 121°C for 15 minutes
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Q. What is the primary component of blood that affects its flow properties?
-
A.
Red blood cells
-
B.
White blood cells
-
C.
Platelets
-
D.
Plasma
Solution
Red blood cells are the primary component that affects blood viscosity and flow properties.
Correct Answer:
A
— Red blood cells
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Q. What is the primary driving force for fluid flow in the circulatory system?
-
A.
Osmotic pressure
-
B.
Hydrostatic pressure
-
C.
Viscosity
-
D.
Surface tension
Solution
Hydrostatic pressure is the primary driving force for fluid flow in the circulatory system, pushing blood through the vessels.
Correct Answer:
B
— Hydrostatic pressure
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Q. What is the primary function of enzymes in biochemical reactions?
-
A.
To increase the temperature of the reaction
-
B.
To act as a substrate
-
C.
To lower the activation energy
-
D.
To change the equilibrium of the reaction
Solution
Enzymes lower the activation energy required for biochemical reactions, thereby increasing the reaction rate.
Correct Answer:
C
— To lower the activation energy
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