Q. What is the main advantage of using CRISPR-Cas9 in plant gene editing?
A.
High precision
B.
Low cost
C.
No need for selectable markers
D.
All of the above
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Solution
CRISPR-Cas9 offers high precision, is relatively low cost, and can be designed to work without selectable markers.
Correct Answer:
D
— All of the above
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Q. What is the primary method used for gene transfer in plants?
A.
Agrobacterium-mediated transformation
B.
Electroporation
C.
Microinjection
D.
Viral vectors
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Solution
Agrobacterium-mediated transformation is the most widely used method for gene transfer in plants due to its efficiency and ability to integrate genes into the plant genome.
Correct Answer:
A
— Agrobacterium-mediated transformation
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Q. What is the purpose of using a binary vector in plant transformation?
A.
To increase transformation efficiency
B.
To simplify cloning procedures
C.
To allow for multiple gene insertions
D.
To enhance plant growth
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Solution
Binary vectors allow for the insertion of multiple genes, facilitating complex traits to be introduced into plants.
Correct Answer:
C
— To allow for multiple gene insertions
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Q. What is the role of the virulence genes in Agrobacterium?
A.
To promote plant growth
B.
To facilitate T-DNA transfer
C.
To enhance photosynthesis
D.
To provide disease resistance
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Solution
Virulence genes in Agrobacterium are responsible for facilitating the transfer of T-DNA into the plant cell.
Correct Answer:
B
— To facilitate T-DNA transfer
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Q. What role does the T-DNA region play in Agrobacterium-mediated gene transfer?
A.
It carries the gene of interest
B.
It provides antibiotic resistance
C.
It facilitates cell division
D.
It enhances photosynthesis
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Solution
The T-DNA region of the Agrobacterium plasmid carries the gene of interest that is transferred into the plant cell.
Correct Answer:
A
— It carries the gene of interest
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Q. Which of the following is a common application of gene transfer in plants?
A.
Improving drought resistance
B.
Enhancing flavor
C.
Increasing yield
D.
All of the above
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Solution
Gene transfer techniques are used for various applications, including improving drought resistance, enhancing flavor, and increasing yield.
Correct Answer:
D
— All of the above
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Q. Which of the following is a common selectable marker used in plant gene transfer?
A.
Kanamycin resistance
B.
Ampicillin resistance
C.
Tetracycline resistance
D.
Streptomycin resistance
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Solution
Kanamycin resistance is commonly used as a selectable marker in plant gene transfer to identify successfully transformed cells.
Correct Answer:
A
— Kanamycin resistance
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Q. Which of the following is a disadvantage of using gene guns for plant transformation?
A.
Low transformation efficiency
B.
Requires specific plant species
C.
Limited to monocots
D.
Expensive equipment
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Solution
Gene guns often have low transformation efficiency compared to other methods like Agrobacterium-mediated transformation.
Correct Answer:
A
— Low transformation efficiency
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Q. Which plant tissue is most commonly used for transformation via Agrobacterium?
A.
Meristematic tissue
B.
Callus tissue
C.
Xylem tissue
D.
Phloem tissue
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Solution
Callus tissue is commonly used for transformation because it can regenerate into whole plants and is more amenable to transformation.
Correct Answer:
B
— Callus tissue
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