Q. What feature do cloning vectors typically contain to facilitate selection?
-
A.
Antibiotic resistance genes
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B.
Introns
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C.
Exons
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D.
Promoters
Solution
Cloning vectors usually contain antibiotic resistance genes to allow for the selection of successfully transformed cells.
Correct Answer:
A
— Antibiotic resistance genes
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Q. What is a key advantage of using yeast artificial chromosomes (YACs) as cloning vectors?
-
A.
Ability to clone large DNA fragments
-
B.
High transformation efficiency
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C.
Low cost
-
D.
Simple construction
Solution
Yeast artificial chromosomes (YACs) can clone large DNA fragments, making them useful for genomic studies.
Correct Answer:
A
— Ability to clone large DNA fragments
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Q. What is the primary function of a cloning vector?
-
A.
To amplify DNA sequences
-
B.
To degrade RNA
-
C.
To synthesize proteins
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D.
To inhibit gene expression
Solution
Cloning vectors are used primarily to amplify specific DNA sequences.
Correct Answer:
A
— To amplify DNA sequences
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Q. What is the purpose of multiple cloning sites (MCS) in a cloning vector?
-
A.
To allow for the insertion of multiple genes
-
B.
To provide a site for replication
-
C.
To enhance stability
-
D.
To facilitate easy insertion of DNA fragments
Solution
Multiple cloning sites (MCS) provide several unique restriction sites for the easy insertion of DNA fragments.
Correct Answer:
D
— To facilitate easy insertion of DNA fragments
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Q. What is the role of a promoter in a cloning vector?
-
A.
To initiate transcription
-
B.
To terminate transcription
-
C.
To replicate DNA
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D.
To bind ribosomes
Solution
A promoter in a cloning vector is essential for initiating the transcription of the inserted gene.
Correct Answer:
A
— To initiate transcription
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Q. Which enzyme is commonly used to insert DNA fragments into cloning vectors?
-
A.
Ligase
-
B.
Polymerase
-
C.
Restriction enzyme
-
D.
Helicase
Solution
DNA ligase is commonly used to insert DNA fragments into cloning vectors by joining the DNA ends.
Correct Answer:
A
— Ligase
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Q. Which of the following is a common type of cloning vector?
-
A.
Plasmids
-
B.
Ribosomes
-
C.
Chromosomes
-
D.
Nucleotides
Solution
Plasmids are one of the most common types of cloning vectors used in molecular biology.
Correct Answer:
A
— Plasmids
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Q. Which of the following is NOT a characteristic of a good cloning vector?
-
A.
High copy number
-
B.
Large size
-
C.
Multiple cloning sites
-
D.
Selectable markers
Solution
A good cloning vector should be of a manageable size; large size can complicate cloning.
Correct Answer:
B
— Large size
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Q. Which type of cloning vector is derived from bacteriophages?
-
A.
Cosmids
-
B.
Bacterial artificial chromosomes
-
C.
Yeast artificial chromosomes
-
D.
Plasmids
Solution
Cosmids are a type of cloning vector that is derived from bacteriophages.
Correct Answer:
A
— Cosmids
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