Q. What is the main advantage of using gas chromatography over liquid chromatography?
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A.
Higher sensitivity for volatile compounds
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B.
Lower cost
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C.
Easier sample preparation
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D.
Wider range of analytes
Solution
Gas chromatography offers higher sensitivity for volatile compounds compared to liquid chromatography.
Correct Answer:
A
— Higher sensitivity for volatile compounds
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Q. What is the primary purpose of gas chromatography in clinical laboratories?
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A.
To separate and analyze volatile compounds
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B.
To measure blood glucose levels
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C.
To perform DNA sequencing
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D.
To culture bacteria
Solution
Gas chromatography is primarily used to separate and analyze volatile compounds in various samples.
Correct Answer:
A
— To separate and analyze volatile compounds
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Q. What is the typical mobile phase used in gas chromatography?
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A.
Liquid solvent
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B.
Gas
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C.
Solid adsorbent
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D.
Aqueous solution
Solution
The mobile phase in gas chromatography is typically a gas, often helium or nitrogen.
Correct Answer:
B
— Gas
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Q. What is the typical temperature range for the column in gas chromatography?
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A.
0-50°C
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B.
50-300°C
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C.
300-500°C
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D.
Above 500°C
Solution
The typical temperature range for the column in gas chromatography is 50-300°C.
Correct Answer:
B
— 50-300°C
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Q. What type of detector is commonly used in gas chromatography for detecting organic compounds?
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A.
Flame ionization detector
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B.
Mass spectrometer
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C.
Ultraviolet detector
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D.
Electrochemical detector
Solution
The flame ionization detector is commonly used for detecting organic compounds in gas chromatography.
Correct Answer:
A
— Flame ionization detector
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Q. Which component of the gas chromatography system is responsible for vaporizing the sample?
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A.
Injector
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B.
Column
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C.
Detector
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D.
Carrier gas
Solution
The injector is responsible for vaporizing the sample before it enters the column.
Correct Answer:
A
— Injector
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Q. Which factor can affect the resolution of a gas chromatography separation?
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A.
Column length
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B.
Temperature of the detector
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C.
Type of carrier gas
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D.
All of the above
Solution
All of the above factors can affect the resolution of a gas chromatography separation.
Correct Answer:
D
— All of the above
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Q. Which of the following is a common application of gas chromatography in medicine?
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A.
Analyzing blood alcohol levels
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B.
Performing MRI scans
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C.
Conducting X-ray imaging
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D.
Measuring blood pressure
Solution
Gas chromatography is commonly used to analyze blood alcohol levels in clinical settings.
Correct Answer:
A
— Analyzing blood alcohol levels
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Q. Which of the following is NOT a common type of gas chromatography?
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A.
Capillary gas chromatography
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B.
Packed column gas chromatography
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C.
Supercritical fluid chromatography
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D.
Gas-solid chromatography
Solution
Supercritical fluid chromatography is not a type of gas chromatography; it uses supercritical fluids as the mobile phase.
Correct Answer:
C
— Supercritical fluid chromatography
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