A.
A type of virus
B.
A circular DNA molecule
C.
A protein structure
D.
A type of RNA
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Solution
A plasmid is a circular DNA molecule that is commonly used as a vector in recombinant DNA technology.
Correct Answer:
B
— A circular DNA molecule
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Q. What is the primary purpose of recombinant DNA technology?
A.
To create genetically modified organisms
B.
To clone human organs
C.
To sequence human genomes
D.
To diagnose genetic disorders
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Solution
Recombinant DNA technology is primarily used to create genetically modified organisms by combining DNA from different sources.
Correct Answer:
A
— To create genetically modified organisms
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Q. What is the purpose of using a selectable marker in recombinant DNA technology?
A.
To enhance protein expression
B.
To identify successfully transformed cells
C.
To increase DNA stability
D.
To reduce mutation rates
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Solution
Selectable markers are used to identify successfully transformed cells that have taken up the recombinant DNA.
Correct Answer:
B
— To identify successfully transformed cells
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Q. What is the role of reverse transcriptase in recombinant DNA technology?
A.
To synthesize DNA from RNA
B.
To cut DNA at specific sites
C.
To ligate DNA fragments
D.
To amplify DNA sequences
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Solution
Reverse transcriptase is an enzyme that synthesizes DNA from RNA, which is important for creating complementary DNA (cDNA) in recombinant DNA technology.
Correct Answer:
A
— To synthesize DNA from RNA
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Q. What is the significance of the 'gene of interest' in recombinant DNA technology?
A.
It is the DNA sequence that is cloned
B.
It is the vector used for cloning
C.
It is the enzyme used for cutting DNA
D.
It is the host organism
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Solution
The 'gene of interest' is the specific DNA sequence that researchers aim to clone and manipulate in recombinant DNA technology.
Correct Answer:
A
— It is the DNA sequence that is cloned
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Q. What role does DNA ligase play in recombinant DNA technology?
A.
It cuts DNA into fragments
B.
It synthesizes RNA
C.
It joins DNA fragments together
D.
It unwinds the DNA helix
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Solution
DNA ligase is an enzyme that joins DNA fragments together, which is crucial for creating recombinant DNA.
Correct Answer:
C
— It joins DNA fragments together
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Q. Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?
A.
DNA polymerase
B.
Ligase
C.
Restriction endonuclease
D.
Helicase
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Solution
Restriction endonucleases are enzymes that cut DNA at specific sequences, which is essential for recombinant DNA technology.
Correct Answer:
C
— Restriction endonuclease
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Q. Which of the following techniques is used to introduce recombinant DNA into host cells?
A.
Electroporation
B.
PCR
C.
Gel electrophoresis
D.
Western blotting
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Solution
Electroporation is a technique used to introduce recombinant DNA into host cells by applying an electrical field to increase cell permeability.
Correct Answer:
A
— Electroporation
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Q. Which organism is often used as a host for cloning in recombinant DNA technology?
A.
Bacteria
B.
Fungi
C.
Plants
D.
Animals
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Solution
Bacteria, particularly E. coli, are commonly used as hosts for cloning in recombinant DNA technology due to their rapid growth and ease of manipulation.
Correct Answer:
A
— Bacteria
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