Recombinant DNA Technology

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Q. What is a plasmid?
  • A. A type of virus
  • B. A circular DNA molecule
  • C. A protein structure
  • D. A type of RNA
Q. What is the primary purpose of recombinant DNA technology?
  • A. To create genetically modified organisms
  • B. To clone human organs
  • C. To sequence human genomes
  • D. To diagnose genetic disorders
Q. What is the purpose of using a selectable marker in recombinant DNA technology?
  • A. To enhance protein expression
  • B. To identify successfully transformed cells
  • C. To increase DNA stability
  • D. To reduce mutation rates
Q. What is the role of reverse transcriptase in recombinant DNA technology?
  • A. To synthesize DNA from RNA
  • B. To cut DNA at specific sites
  • C. To ligate DNA fragments
  • D. To amplify DNA sequences
Q. What is the significance of the 'gene of interest' in recombinant DNA technology?
  • A. It is the DNA sequence that is cloned
  • B. It is the vector used for cloning
  • C. It is the enzyme used for cutting DNA
  • D. It is the host organism
Q. What role does DNA ligase play in recombinant DNA technology?
  • A. It cuts DNA into fragments
  • B. It synthesizes RNA
  • C. It joins DNA fragments together
  • D. It unwinds the DNA helix
Q. Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?
  • A. DNA polymerase
  • B. Ligase
  • C. Restriction endonuclease
  • D. Helicase
Q. Which of the following techniques is used to introduce recombinant DNA into host cells?
  • A. Electroporation
  • B. PCR
  • C. Gel electrophoresis
  • D. Western blotting
Q. Which organism is often used as a host for cloning in recombinant DNA technology?
  • A. Bacteria
  • B. Fungi
  • C. Plants
  • D. Animals
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