Q. What is the main advantage of using high-performance liquid chromatography (HPLC) for protein purification?
-
A.
It is less expensive
-
B.
It provides high resolution and speed
-
C.
It requires less sample
-
D.
It is easier to set up
Solution
HPLC provides high resolution and speed, making it a powerful technique for protein purification.
Correct Answer:
B
— It provides high resolution and speed
Learn More →
Q. What is the primary purpose of protein purification?
-
A.
To increase protein concentration
-
B.
To isolate a specific protein from a mixture
-
C.
To denature proteins
-
D.
To enhance protein activity
Solution
The primary purpose of protein purification is to isolate a specific protein from a mixture for further study or use.
Correct Answer:
B
— To isolate a specific protein from a mixture
Learn More →
Q. What is the purpose of using SDS-PAGE in protein analysis?
-
A.
To determine protein concentration
-
B.
To separate proteins based on size
-
C.
To identify protein sequences
-
D.
To purify proteins
Solution
SDS-PAGE is used to separate proteins based on their size, allowing for analysis of protein purity and molecular weight.
Correct Answer:
B
— To separate proteins based on size
Learn More →
Q. What is the role of affinity chromatography in protein purification?
-
A.
To separate proteins based on size
-
B.
To separate proteins based on charge
-
C.
To isolate proteins that bind to specific ligands
-
D.
To denature proteins for analysis
Solution
Affinity chromatography is used to isolate proteins that specifically bind to certain ligands, allowing for targeted purification.
Correct Answer:
C
— To isolate proteins that bind to specific ligands
Learn More →
Q. What is the significance of using a buffer during protein purification?
-
A.
To increase protein solubility
-
B.
To maintain pH and ionic strength
-
C.
To enhance protein activity
-
D.
To precipitate unwanted proteins
Solution
Buffers are used during protein purification to maintain pH and ionic strength, which are critical for protein stability and solubility.
Correct Answer:
B
— To maintain pH and ionic strength
Learn More →
Q. Which factor can affect the yield of protein purification?
-
A.
Temperature
-
B.
pH
-
C.
Choice of purification method
-
D.
All of the above
Solution
All of the above factors can significantly affect the yield of protein purification.
Correct Answer:
D
— All of the above
Learn More →
Q. Which method can be used to determine the purity of a protein sample?
-
A.
Mass spectrometry
-
B.
Western blotting
-
C.
Both A and B
-
D.
None of the above
Solution
Both mass spectrometry and Western blotting can be used to assess the purity of a protein sample.
Correct Answer:
C
— Both A and B
Learn More →
Q. Which of the following is a common method for concentrating proteins?
-
A.
Gel filtration
-
B.
Ultrafiltration
-
C.
Ion exchange
-
D.
Affinity chromatography
Solution
Ultrafiltration is a common method used to concentrate proteins by removing excess solvent.
Correct Answer:
B
— Ultrafiltration
Learn More →
Q. Which technique is commonly used for the initial step in protein purification?
-
A.
Dialysis
-
B.
Precipitation
-
C.
Chromatography
-
D.
Electrophoresis
Solution
Precipitation is often used as an initial step in protein purification to concentrate proteins and remove contaminants.
Correct Answer:
B
— Precipitation
Learn More →
Showing 1 to 9 of 9 (1 Pages)
