Q. What are the three main steps of the PCR cycle?
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A.
Denaturation, annealing, extension
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B.
Denaturation, transcription, translation
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C.
Annealing, elongation, termination
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D.
Denaturation, replication, repair
Solution
The three main steps of PCR are denaturation, annealing, and extension.
Correct Answer:
A
— Denaturation, annealing, extension
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Q. What is a potential limitation of PCR?
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A.
It can amplify only DNA
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B.
It requires high-quality DNA
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C.
It is a slow process
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D.
It cannot be automated
Solution
PCR requires high-quality DNA as contaminants can inhibit the reaction and affect results.
Correct Answer:
B
— It requires high-quality DNA
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Q. Which type of PCR is used to amplify RNA?
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A.
Real-time PCR
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B.
Reverse transcription PCR
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C.
Multiplex PCR
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D.
Nested PCR
Solution
Reverse transcription PCR (RT-PCR) is used to amplify RNA by first converting it to complementary DNA (cDNA).
Correct Answer:
B
— Reverse transcription PCR
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