Q. What is a common method for sterilizing cell culture media?
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A.
Autoclaving
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B.
Filtration
-
C.
Chemical sterilization
-
D.
All of the above
Solution
All of the listed methods can be used to sterilize cell culture media to prevent contamination.
Correct Answer:
D
— All of the above
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Q. What is the primary purpose of cell culture in biomedical research?
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A.
To study the effects of drugs on whole organisms
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B.
To grow cells in a controlled environment for experimentation
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C.
To replace animal testing entirely
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D.
To produce food products
Solution
Cell culture allows researchers to grow cells in a controlled environment, facilitating various experiments.
Correct Answer:
B
— To grow cells in a controlled environment for experimentation
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Q. What is the purpose of using a CO2 incubator in cell culture?
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A.
To maintain a sterile environment
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B.
To control temperature and humidity
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C.
To provide a controlled CO2 atmosphere for optimal pH
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D.
To enhance cell growth
Solution
A CO2 incubator maintains a controlled CO2 atmosphere, which is crucial for maintaining the pH of the culture medium.
Correct Answer:
C
— To provide a controlled CO2 atmosphere for optimal pH
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Q. What is the role of serum in cell culture media?
-
A.
To provide a source of nutrients and growth factors
-
B.
To act as a buffer
-
C.
To prevent contamination
-
D.
To enhance cell adhesion
Solution
Serum provides essential nutrients, hormones, and growth factors necessary for cell growth and maintenance.
Correct Answer:
A
— To provide a source of nutrients and growth factors
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Q. What is the significance of the 'passage number' in cell culture?
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A.
It indicates the age of the cells
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B.
It refers to the number of times cells have been subcultured
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C.
It measures cell viability
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D.
It determines the growth rate of the cells
Solution
The passage number indicates how many times the cells have been subcultured, which is important for maintaining cell characteristics.
Correct Answer:
B
— It refers to the number of times cells have been subcultured
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Q. Which of the following is a characteristic of immortalized cell lines?
-
A.
They can only divide a limited number of times
-
B.
They have a finite lifespan
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C.
They can proliferate indefinitely
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D.
They are always derived from human tissues
Solution
Immortalized cell lines can proliferate indefinitely, making them useful for long-term studies.
Correct Answer:
C
— They can proliferate indefinitely
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Q. Which of the following is NOT a common contaminant in cell cultures?
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A.
Bacteria
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B.
Fungi
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C.
Viruses
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D.
Nutrients
Solution
Nutrients are essential for cell growth, while bacteria, fungi, and viruses are common contaminants.
Correct Answer:
D
— Nutrients
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Q. Which type of cell culture involves the growth of cells from a single cell type?
-
A.
Primary culture
-
B.
Secondary culture
-
C.
Clonal culture
-
D.
Mixed culture
Solution
Clonal culture refers to the growth of cells derived from a single cell, ensuring uniformity in the cell population.
Correct Answer:
C
— Clonal culture
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Q. Which type of cell culture is best for studying the interactions between different cell types?
-
A.
Primary culture
-
B.
Clonal culture
-
C.
Mixed culture
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D.
Suspension culture
Solution
Mixed culture allows for the study of interactions between different cell types, providing insights into cellular communication.
Correct Answer:
C
— Mixed culture
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Q. Which type of cell culture is typically used for the production of monoclonal antibodies?
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A.
Primary culture
-
B.
Suspension culture
-
C.
Adherent culture
-
D.
3D culture
Solution
Suspension culture is commonly used for the production of monoclonal antibodies as it allows for high-density cell growth.
Correct Answer:
B
— Suspension culture
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