Cell Cultures and Characteristics

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Q. What is a common method for sterilizing cell culture media?
  • A. Autoclaving
  • B. Filtration
  • C. Chemical sterilization
  • D. All of the above
Q. What is the primary purpose of cell culture in biomedical research?
  • A. To study the effects of drugs on whole organisms
  • B. To grow cells in a controlled environment for experimentation
  • C. To replace animal testing entirely
  • D. To produce food products
Q. What is the purpose of using a CO2 incubator in cell culture?
  • A. To maintain a sterile environment
  • B. To control temperature and humidity
  • C. To provide a controlled CO2 atmosphere for optimal pH
  • D. To enhance cell growth
Q. What is the role of serum in cell culture media?
  • A. To provide a source of nutrients and growth factors
  • B. To act as a buffer
  • C. To prevent contamination
  • D. To enhance cell adhesion
Q. What is the significance of the 'passage number' in cell culture?
  • A. It indicates the age of the cells
  • B. It refers to the number of times cells have been subcultured
  • C. It measures cell viability
  • D. It determines the growth rate of the cells
Q. Which of the following is a characteristic of immortalized cell lines?
  • A. They can only divide a limited number of times
  • B. They have a finite lifespan
  • C. They can proliferate indefinitely
  • D. They are always derived from human tissues
Q. Which of the following is NOT a common contaminant in cell cultures?
  • A. Bacteria
  • B. Fungi
  • C. Viruses
  • D. Nutrients
Q. Which type of cell culture involves the growth of cells from a single cell type?
  • A. Primary culture
  • B. Secondary culture
  • C. Clonal culture
  • D. Mixed culture
Q. Which type of cell culture is best for studying the interactions between different cell types?
  • A. Primary culture
  • B. Clonal culture
  • C. Mixed culture
  • D. Suspension culture
Q. Which type of cell culture is typically used for the production of monoclonal antibodies?
  • A. Primary culture
  • B. Suspension culture
  • C. Adherent culture
  • D. 3D culture
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