Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. Which application is NOT commonly associated with PCR?
A.
Genetic testing
B.
Forensic analysis
C.
Protein synthesis
D.
Pathogen detection
Show solution
Solution
PCR is not used for protein synthesis; it is primarily used for amplifying DNA for various applications.
Correct Answer:
C
— Protein synthesis
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Q. Which autoimmune disease is characterized by dry eyes and dry mouth due to the immune system attacking exocrine glands?
A.
Sjögren's Syndrome
B.
Rheumatoid Arthritis
C.
Systemic Lupus Erythematosus
D.
Ankylosing Spondylitis
Show solution
Solution
Sjögren's Syndrome is an autoimmune disease where the immune system attacks the glands that produce moisture, leading to dry eyes and mouth.
Correct Answer:
A
— Sjögren's Syndrome
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Q. Which autoimmune disease is characterized by the presence of anti-citrullinated protein antibodies (ACPAs)?
A.
Rheumatoid Arthritis
B.
Systemic Lupus Erythematosus
C.
Ankylosing Spondylitis
D.
Psoriatic Arthritis
Show solution
Solution
Rheumatoid Arthritis is often associated with the presence of anti-citrullinated protein antibodies (ACPAs), which are used as a diagnostic marker.
Correct Answer:
A
— Rheumatoid Arthritis
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Q. Which autoimmune disorder is associated with the overproduction of thyroid hormones?
A.
Hashimoto's Thyroiditis
B.
Graves' Disease
C.
Sjögren's Syndrome
D.
Celiac Disease
Show solution
Solution
Graves' Disease is an autoimmune disorder that leads to the overproduction of thyroid hormones due to the immune system's attack on the thyroid gland.
Correct Answer:
B
— Graves' Disease
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Q. Which base pairs with adenine in DNA?
A.
Thymine
B.
Cytosine
C.
Guanine
D.
Uracil
Show solution
Solution
In DNA, adenine pairs with thymine through hydrogen bonds.
Correct Answer:
A
— Thymine
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Q. Which bond is responsible for the primary structure of peptides?
A.
Hydrogen bonds
B.
Ionic bonds
C.
Peptide bonds
D.
Disulfide bonds
Show solution
Solution
Peptide bonds are covalent bonds that link amino acids together to form the primary structure of peptides.
Correct Answer:
C
— Peptide bonds
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Q. Which cells are primarily involved in the humoral immune response?
A.
T cells
B.
B cells
C.
Macrophages
D.
Natural killer cells
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Solution
B cells are responsible for the humoral immune response, producing antibodies that target specific antigens.
Correct Answer:
B
— B cells
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Q. Which cells are primarily involved in the innate immune response?
A.
B cells
B.
T cells
C.
Macrophages
D.
Plasma cells
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Solution
Macrophages are key players in the innate immune response, providing immediate defense against pathogens.
Correct Answer:
C
— Macrophages
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Q. Which complex in the electron transport chain is responsible for pumping protons into the intermembrane space?
A.
Complex I
B.
Complex II
C.
Complex III
D.
Complex IV
Show solution
Solution
Complex I (NADH dehydrogenase) pumps protons into the intermembrane space.
Correct Answer:
A
— Complex I
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Q. Which component is essential for a UV luminance spectroscopy setup?
A.
Spectrophotometer
B.
Fluorometer
C.
Chromatograph
D.
Microscope
Show solution
Solution
A spectrophotometer is essential for a UV luminance spectroscopy setup, as it measures the intensity of light absorbed by the sample.
Correct Answer:
A
— Spectrophotometer
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Q. Which component is essential for the separation process in HPLC?
A.
Detector
B.
Mobile phase
C.
Sample injector
D.
Column
Show solution
Solution
The column is essential for the separation process in HPLC, as it contains the stationary phase where the separation occurs.
Correct Answer:
D
— Column
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Q. Which component of chromatography is responsible for the separation of compounds?
A.
Mobile phase
B.
Stationary phase
C.
Detector
D.
Sample injector
Show solution
Solution
The stationary phase is responsible for the separation of compounds in chromatography.
Correct Answer:
B
— Stationary phase
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Q. Which component of FTIR spectroscopy is responsible for detecting the infrared light?
A.
Laser
B.
Photodetector
C.
Sample holder
D.
Computer
Show solution
Solution
The photodetector is responsible for detecting the infrared light after it interacts with the sample in FTIR spectroscopy.
Correct Answer:
B
— Photodetector
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Q. Which component of the cell membrane is primarily responsible for fluidity?
A.
Cholesterol
B.
Proteins
C.
Carbohydrates
D.
Phospholipids
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Solution
Cholesterol molecules intercalate between phospholipids, maintaining membrane fluidity across varying temperatures.
Correct Answer:
A
— Cholesterol
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Q. Which component of the cell membrane is primarily responsible for its fluidity?
A.
Proteins
B.
Carbohydrates
C.
Phospholipids
D.
Cholesterol
Show solution
Solution
Cholesterol molecules interspersed within the phospholipid bilayer help maintain membrane fluidity.
Correct Answer:
D
— Cholesterol
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Q. Which component of the gas chromatography system is responsible for vaporizing the sample?
A.
Injector
B.
Column
C.
Detector
D.
Carrier gas
Show solution
Solution
The injector is responsible for vaporizing the sample before it enters the column.
Correct Answer:
A
— Injector
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Q. Which compound is regenerated at the end of the TCA cycle?
A.
Acetyl-CoA
B.
Citrate
C.
Oxaloacetate
D.
Alpha-ketoglutarate
Show solution
Solution
Oxaloacetate is regenerated at the end of the TCA cycle, allowing the cycle to continue.
Correct Answer:
C
— Oxaloacetate
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Q. Which condition can inhibit oxidative phosphorylation?
A.
High oxygen levels
B.
Low glucose levels
C.
Presence of cyanide
D.
High ATP levels
Show solution
Solution
Cyanide inhibits Complex IV of the electron transport chain, blocking oxidative phosphorylation.
Correct Answer:
C
— Presence of cyanide
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Q. Which condition can lead to protein aggregation and loss of function?
A.
High salt concentration
B.
Low pH
C.
High temperature
D.
All of the above
Show solution
Solution
All of these conditions can lead to protein aggregation and loss of function.
Correct Answer:
D
— All of the above
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Q. Which cytokine is primarily involved in the regulation of immune responses?
A.
Interferon
B.
Histamine
C.
Prostaglandin
D.
Serotonin
Show solution
Solution
Interferons are key cytokines that help regulate immune responses, particularly in viral infections.
Correct Answer:
A
— Interferon
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Q. Which cytokine is primarily responsible for activating T cells?
A.
Interleukin-1
B.
Interleukin-2
C.
Tumor necrosis factor
D.
Interferon-gamma
Show solution
Solution
Interleukin-2 is crucial for T cell activation and proliferation during the immune response.
Correct Answer:
B
— Interleukin-2
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Q. Which disease is characterized by chronic inflammation of the joints and is classified as an autoimmune disorder?
A.
Rheumatoid Arthritis
B.
Osteoarthritis
C.
Gout
D.
Fibromyalgia
Show solution
Solution
Rheumatoid Arthritis is an autoimmune disease that causes chronic inflammation of the joints.
Correct Answer:
A
— Rheumatoid Arthritis
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Q. Which disease is primarily caused by the destruction of insulin-producing beta cells in the pancreas?
A.
Celiac Disease
B.
Type 1 Diabetes
C.
Graves' Disease
D.
Hashimoto's Thyroiditis
Show solution
Solution
Type 1 Diabetes is an autoimmune condition where the immune system attacks and destroys the insulin-producing beta cells in the pancreas.
Correct Answer:
B
— Type 1 Diabetes
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Q. Which enzyme catalyzes the conversion of citrate to isocitrate?
A.
Citrate synthase
B.
Aconitase
C.
Isocitrate dehydrogenase
D.
Alpha-ketoglutarate dehydrogenase
Show solution
Solution
Aconitase catalyzes the conversion of citrate to isocitrate in the TCA cycle.
Correct Answer:
B
— Aconitase
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Q. Which enzyme catalyzes the conversion of glucose to glucose-6-phosphate in glycolysis?
A.
Hexokinase
B.
Phosphofructokinase
C.
Pyruvate kinase
D.
Aldolase
Show solution
Solution
Hexokinase catalyzes the phosphorylation of glucose to glucose-6-phosphate.
Correct Answer:
A
— Hexokinase
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Q. Which enzyme catalyzes the first step of the urea cycle?
A.
Carbamoyl phosphate synthetase
B.
Ornithine transcarbamylase
C.
Arginase
D.
Argininosuccinate synthetase
Show solution
Solution
Carbamoyl phosphate synthetase catalyzes the first step of the urea cycle, converting ammonia and bicarbonate to carbamoyl phosphate.
Correct Answer:
A
— Carbamoyl phosphate synthetase
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Q. Which enzyme initiates the process of fatty acid oxidation?
A.
Acetyl-CoA carboxylase
B.
Carnitine acyltransferase
C.
Fatty acyl-CoA synthetase
D.
Beta-hydroxyacyl-CoA dehydrogenase
Show solution
Solution
Fatty acyl-CoA synthetase initiates fatty acid oxidation by converting fatty acids into fatty acyl-CoA.
Correct Answer:
C
— Fatty acyl-CoA synthetase
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Q. Which enzyme is commonly used to cut DNA at specific sequences in recombinant DNA technology?
A.
DNA polymerase
B.
Ligase
C.
Restriction endonuclease
D.
Helicase
Show solution
Solution
Restriction endonucleases are enzymes that cut DNA at specific sequences, which is essential for recombinant DNA technology.
Correct Answer:
C
— Restriction endonuclease
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Q. Which enzyme is involved in the digestion of carbohydrates?
A.
Pepsin
B.
Amylase
C.
Lipase
D.
Trypsin
Show solution
Solution
Amylase is the enzyme that breaks down carbohydrates into simpler sugars.
Correct Answer:
B
— Amylase
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Q. Which enzyme is responsible for breaking down hydrogen peroxide in cells?
A.
Catalase
B.
Amylase
C.
Lipase
D.
Protease
Show solution
Solution
Catalase is the enzyme that catalyzes the decomposition of hydrogen peroxide into water and oxygen.
Correct Answer:
A
— Catalase
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