Biotechnology MCQ & Objective Questions
Biotechnology is a crucial subject for students preparing for school and competitive exams in India. Understanding its concepts can significantly enhance your performance. Practicing MCQs and objective questions in Biotechnology not only helps in reinforcing your knowledge but also boosts your confidence in tackling important questions during exams.
What You Will Practise Here
Fundamentals of Genetic Engineering
Applications of Biotechnology in Medicine
Biotechnological Techniques: PCR, Cloning, and Sequencing
Microbial Biotechnology and its Industrial Applications
Plant Biotechnology: GMOs and Tissue Culture
Ethical Issues in Biotechnology
Important Definitions and Key Formulas
Exam Relevance
Biotechnology is a significant topic in various examinations, including CBSE, State Boards, NEET, and JEE. Students can expect questions related to the applications of biotechnology, techniques used in genetic engineering, and ethical considerations. Common patterns include multiple-choice questions that test conceptual understanding and application of theories in real-world scenarios.
Common Mistakes Students Make
Confusing different biotechnological techniques and their applications.
Overlooking ethical implications and regulations related to biotechnology.
Misunderstanding the role of enzymes in biotechnological processes.
Failing to connect theoretical concepts with practical applications.
FAQs
Question: What are some key areas to focus on in Biotechnology for exams?Answer: Focus on genetic engineering techniques, applications in medicine, and ethical issues.
Question: How can I improve my performance in Biotechnology MCQs?Answer: Regular practice of objective questions and understanding key concepts will help you score better.
Don't wait any longer! Start solving Biotechnology MCQs today to test your understanding and prepare effectively for your exams. Your success in mastering this subject is just a practice question away!
Q. What is the significance of the CRISPR-Cas9 technology in plant genetics?
A.
It allows for the cloning of plants
B.
It enables precise gene editing
C.
It increases plant growth rate
D.
It enhances photosynthetic efficiency
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Solution
CRISPR-Cas9 is a revolutionary tool that allows for precise editing of plant genomes.
Correct Answer:
B
— It enables precise gene editing
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Q. What is the significance of the nitrogen cycle in ecosystems?
A.
It helps in energy transfer
B.
It recycles nutrients
C.
It increases carbon dioxide levels
D.
It reduces soil acidity
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Solution
The nitrogen cycle is crucial for recycling nitrogen in various forms, making it available for plant use.
Correct Answer:
B
— It recycles nutrients
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Q. What is the significance of the polymerase chain reaction (PCR) in recombinant DNA technology?
A.
It cuts DNA into fragments
B.
It amplifies specific DNA sequences
C.
It joins DNA fragments
D.
It sequences DNA
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Solution
PCR is significant because it amplifies specific DNA sequences, making it easier to work with small amounts of DNA.
Correct Answer:
B
— It amplifies specific DNA sequences
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Q. What is the term for animals that have had their genome altered by the transfer of a gene from another species?
A.
Cloned animals
B.
Transgenic animals
C.
Hybrid animals
D.
Genetically modified organisms
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Solution
Transgenic animals are those that have had their genome altered by the transfer of a gene from another species.
Correct Answer:
B
— Transgenic animals
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Q. What is the term for the process of creating genetically modified animals?
A.
Cloning
B.
Transgenesis
C.
Selective breeding
D.
Hybridization
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Solution
Transgenesis is the process of introducing foreign DNA into an animal's genome to create genetically modified organisms.
Correct Answer:
B
— Transgenesis
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Q. What is the term for the process of growing plant cells in a controlled environment?
A.
Hydroponics
B.
Tissue culture
C.
Aeroponics
D.
Xenobiotics
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Solution
Tissue culture is the process of growing plant cells in a controlled environment.
Correct Answer:
B
— Tissue culture
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Q. What is the typical alcohol content of fermented beverages produced through ethanol fermentation?
A.
1-5%
B.
5-15%
C.
15-25%
D.
25-40%
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Solution
The typical alcohol content of fermented beverages produced through ethanol fermentation ranges from 5-15%.
Correct Answer:
B
— 5-15%
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Q. What is the typical concentration of acetic acid in household vinegar?
A.
5-20%
B.
1-3%
C.
30-50%
D.
50-70%
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Solution
Household vinegar typically contains 5-20% acetic acid.
Correct Answer:
A
— 5-20%
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Q. What is the typical pH range for animal cell culture media?
A.
4.0 - 5.0
B.
6.5 - 7.5
C.
8.0 - 9.0
D.
10.0 - 11.0
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Solution
The typical pH range for animal cell culture media is 6.5 to 7.5, which is optimal for cell growth and function.
Correct Answer:
B
— 6.5 - 7.5
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Q. What phase of the cell cycle is characterized by DNA replication?
A.
G1 phase
B.
S phase
C.
G2 phase
D.
M phase
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Solution
The S phase is where DNA replication occurs, resulting in the duplication of chromosomes.
Correct Answer:
B
— S phase
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Q. What regulatory body typically oversees the approval of transgenic plants?
A.
Environmental Protection Agency (EPA)
B.
Food and Drug Administration (FDA)
C.
United States Department of Agriculture (USDA)
D.
All of the above
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Solution
In the United States, the approval of transgenic plants is overseen by multiple agencies, including the EPA, FDA, and USDA.
Correct Answer:
D
— All of the above
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Q. What role do enzymes play in the synthesis of therapeutic agents?
A.
They act as catalysts
B.
They inhibit reactions
C.
They are the final product
D.
They are not involved
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Solution
Enzymes act as catalysts to speed up the synthesis of therapeutic agents.
Correct Answer:
A
— They act as catalysts
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Q. What role does agar play in plant cell culture media?
A.
Nutrient source
B.
pH stabilizer
C.
Solidifying agent
D.
Growth regulator
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Solution
Agar serves as a solidifying agent in plant cell culture media, providing a stable surface for cell growth.
Correct Answer:
C
— Solidifying agent
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Q. What role does the T-DNA region play in Agrobacterium-mediated gene transfer?
A.
It carries the gene of interest
B.
It provides antibiotic resistance
C.
It facilitates cell division
D.
It enhances photosynthesis
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Solution
The T-DNA region of the Agrobacterium plasmid carries the gene of interest that is transferred into the plant cell.
Correct Answer:
A
— It carries the gene of interest
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Q. What type of biosensor uses an enzyme as the bioreceptor?
A.
Immunosensor
B.
Enzyme-based biosensor
C.
DNA biosensor
D.
Electrochemical biosensor
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Solution
An enzyme-based biosensor utilizes enzymes as bioreceptors to catalyze reactions with specific substrates, leading to measurable signals.
Correct Answer:
B
— Enzyme-based biosensor
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Q. What type of immune response do monoclonal antibodies primarily elicit?
A.
Innate immune response
B.
Adaptive immune response
C.
Inflammatory response
D.
Autoimmune response
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Solution
Monoclonal antibodies primarily elicit an adaptive immune response by targeting specific antigens.
Correct Answer:
B
— Adaptive immune response
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Q. Which animal is commonly used as a model organism in genetic studies?
A.
Dog
B.
Mouse
C.
Cat
D.
Horse
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Solution
The mouse is widely used as a model organism in genetic studies due to its genetic similarity to humans and short reproductive cycle.
Correct Answer:
B
— Mouse
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Q. Which animal is most commonly used for transgenic research?
A.
Dogs
B.
Cats
C.
Mice
D.
Rabbits
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Solution
Mice are the most commonly used animals in transgenic research due to their genetic similarity to humans and short reproductive cycles.
Correct Answer:
C
— Mice
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Q. Which antibiotic is effective against anaerobic bacteria?
A.
Cefazolin
B.
Metronidazole
C.
Amoxicillin
D.
Azithromycin
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Solution
Metronidazole is effective against anaerobic bacteria and is often used for such infections.
Correct Answer:
B
— Metronidazole
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Q. Which antibiotic is often used to treat MRSA infections?
A.
Ceftriaxone
B.
Clindamycin
C.
Amoxicillin
D.
Gentamicin
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Solution
Clindamycin is commonly used to treat infections caused by MRSA.
Correct Answer:
B
— Clindamycin
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Q. Which biotechnology technique is used to amplify small segments of DNA?
A.
Gene cloning
B.
PCR (Polymerase Chain Reaction)
C.
DNA sequencing
D.
Electrophoresis
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Solution
PCR (Polymerase Chain Reaction) is the technique used to amplify small segments of DNA.
Correct Answer:
B
— PCR (Polymerase Chain Reaction)
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Q. Which checkpoint is crucial for preventing the division of cells with damaged DNA?
A.
G1 checkpoint
B.
S checkpoint
C.
G2 checkpoint
D.
M checkpoint
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Solution
The G2 checkpoint is crucial for preventing the division of cells with damaged DNA by ensuring all DNA is intact before mitosis.
Correct Answer:
C
— G2 checkpoint
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Q. Which component in wine is primarily responsible for its antioxidant properties?
A.
Tannins
B.
Sugars
C.
Acids
D.
Alcohol
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Solution
Tannins are polyphenolic compounds in wine that contribute to its antioxidant properties.
Correct Answer:
A
— Tannins
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Q. Which component is commonly added to plant tissue culture media to promote shoot regeneration?
A.
Agar
B.
Cytokinins
C.
Sucrose
D.
Gibberellins
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Solution
Cytokinins are plant hormones that promote cell division and are commonly used to enhance shoot regeneration in plant tissue culture.
Correct Answer:
B
— Cytokinins
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Q. Which component of a biosensor is responsible for the recognition of the target analyte?
A.
Transducer
B.
Bioreceptor
C.
Signal processor
D.
Display unit
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Solution
The bioreceptor is the component that specifically recognizes and binds to the target analyte in a biosensor.
Correct Answer:
B
— Bioreceptor
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Q. Which disorder involves a fear of social situations?
A.
Agoraphobia
B.
Social anxiety disorder
C.
Specific phobia
D.
Generalized anxiety disorder
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Solution
Social anxiety disorder is characterized by an intense fear of social situations where one may be judged or scrutinized.
Correct Answer:
B
— Social anxiety disorder
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Q. Which disorder is characterized by a fear of being in situations where escape might be difficult?
A.
Panic disorder
B.
Agoraphobia
C.
Social anxiety disorder
D.
Specific phobia
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Solution
Agoraphobia is characterized by a fear of being in situations where escape might be difficult or help unavailable.
Correct Answer:
B
— Agoraphobia
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Q. Which disorder is characterized by episodes of extreme mood swings?
A.
Bipolar disorder
B.
Major depressive disorder
C.
Anxiety disorder
D.
Schizophrenia
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Solution
Bipolar disorder is characterized by episodes of extreme mood swings, including manic and depressive episodes.
Correct Answer:
A
— Bipolar disorder
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Q. Which disorder is characterized by excessive worry about various aspects of life?
A.
Panic disorder
B.
Generalized anxiety disorder
C.
Social anxiety disorder
D.
Post-traumatic stress disorder
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Solution
Generalized anxiety disorder is characterized by excessive and uncontrollable worry about multiple aspects of life.
Correct Answer:
B
— Generalized anxiety disorder
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Q. Which enzyme is commonly used to insert DNA fragments into cloning vectors?
A.
Ligase
B.
Polymerase
C.
Restriction enzyme
D.
Helicase
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Solution
DNA ligase is commonly used to insert DNA fragments into cloning vectors by joining the DNA ends.
Correct Answer:
A
— Ligase
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