Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. What is the primary purpose of the mobile phase in HPLC?
A.
To provide a stationary phase
B.
To dissolve the sample
C.
To detect the analytes
D.
To inject the sample
Show solution
Solution
The primary purpose of the mobile phase in HPLC is to dissolve the sample and carry it through the column.
Correct Answer:
B
— To dissolve the sample
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Q. What is the primary reactant in photosynthesis?
A.
Glucose
B.
Oxygen
C.
Carbon Dioxide
D.
Water
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Solution
Carbon Dioxide is one of the primary reactants in the process of photosynthesis.
Correct Answer:
C
— Carbon Dioxide
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Q. What is the primary role of allosteric enzymes in metabolic pathways?
A.
To catalyze irreversible reactions
B.
To provide feedback inhibition
C.
To enhance substrate binding
D.
To stabilize the transition state
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Solution
Allosteric enzymes often provide feedback inhibition, regulating metabolic pathways based on product concentration.
Correct Answer:
B
— To provide feedback inhibition
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Q. What is the primary role of cholesterol in the cell membrane?
A.
To provide energy
B.
To stabilize membrane fluidity
C.
To facilitate transport
D.
To act as a receptor
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Solution
Cholesterol helps to stabilize the fluidity of the membrane, preventing it from becoming too rigid or too fluid.
Correct Answer:
B
— To stabilize membrane fluidity
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Q. What is the primary role of hemoglobin in the blood?
A.
Transporting nutrients
B.
Regulating pH
C.
Transporting oxygen
D.
Clotting blood
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Solution
Hemoglobin's primary role is to transport oxygen from the lungs to the tissues and facilitate carbon dioxide transport back to the lungs.
Correct Answer:
C
— Transporting oxygen
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Q. What is the primary role of proteins in nucleic acid interactions?
A.
Catalyzing chemical reactions
B.
Storing genetic information
C.
Facilitating transcription and replication
D.
Providing structural support
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Solution
Proteins, such as transcription factors and polymerases, facilitate the processes of transcription and replication by interacting with nucleic acids.
Correct Answer:
C
— Facilitating transcription and replication
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Q. What is the primary role of the plasma membrane?
A.
Energy production
B.
Regulating what enters and exits the cell
C.
Protein synthesis
D.
Storage of genetic information
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Solution
The plasma membrane regulates the movement of substances into and out of the cell, maintaining homeostasis.
Correct Answer:
B
— Regulating what enters and exits the cell
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Q. What is the primary site of amino acid metabolism in the body?
A.
Liver
B.
Kidney
C.
Muscle
D.
Intestine
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Solution
The liver is the primary site for amino acid metabolism, where transamination and deamination occur.
Correct Answer:
A
— Liver
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Q. What is the primary site of ATP synthesis in eukaryotic cells?
A.
Cytoplasm
B.
Mitochondria
C.
Nucleus
D.
Endoplasmic Reticulum
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Solution
ATP synthesis primarily occurs in the mitochondria through oxidative phosphorylation.
Correct Answer:
B
— Mitochondria
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Q. What is the primary source of electrons for the electron transport chain?
A.
Glucose
B.
NADH and FADH2
C.
Oxygen
D.
ATP
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Solution
NADH and FADH2 are the primary sources of electrons for the electron transport chain.
Correct Answer:
B
— NADH and FADH2
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Q. What is the primary source of nitrogen for the synthesis of non-essential amino acids?
A.
Dietary proteins
B.
Ammonia
C.
Urea
D.
Nucleic acids
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Solution
Ammonia is the primary source of nitrogen for the synthesis of non-essential amino acids through transamination.
Correct Answer:
B
— Ammonia
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Q. What is the primary structure of a peptide?
A.
The sequence of amino acids
B.
The three-dimensional shape
C.
The arrangement of beta sheets
D.
The interaction with other peptides
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Solution
The primary structure of a peptide refers to the linear sequence of amino acids linked by peptide bonds.
Correct Answer:
A
— The sequence of amino acids
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Q. What is the primary structure of RNA composed of?
A.
Amino acids
B.
Nucleotides
C.
Fatty acids
D.
Monosaccharides
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Solution
The primary structure of RNA is composed of nucleotides, which are the building blocks of RNA.
Correct Answer:
B
— Nucleotides
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Q. What is the process of copying DNA called?
A.
Transcription
B.
Translation
C.
Replication
D.
Mutation
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Solution
The process of copying DNA is known as replication.
Correct Answer:
C
— Replication
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Q. What is the proton motive force?
A.
The energy from ATP hydrolysis
B.
The gradient of protons across the membrane
C.
The flow of electrons through the chain
D.
The consumption of oxygen
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Solution
The proton motive force is the gradient of protons across the inner mitochondrial membrane that drives ATP synthesis.
Correct Answer:
B
— The gradient of protons across the membrane
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Q. What is the purpose of a DNA ladder in gel electrophoresis?
A.
To visualize the gel
B.
To provide a size reference for DNA fragments
C.
To enhance the resolution of bands
D.
To stain the DNA
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Solution
A DNA ladder serves as a size reference for comparing the lengths of DNA fragments in the gel.
Correct Answer:
B
— To provide a size reference for DNA fragments
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Q. What is the purpose of staining the gel after electrophoresis?
A.
To enhance the gel's structural integrity
B.
To visualize the separated DNA or proteins
C.
To increase the gel's conductivity
D.
To prevent degradation of samples
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Solution
Staining the gel allows for visualization of the separated DNA or proteins after electrophoresis.
Correct Answer:
B
— To visualize the separated DNA or proteins
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Q. What is the purpose of using a secondary antibody in immunological assays?
A.
To bind to the primary antibody
B.
To enhance the immune response
C.
To isolate specific cells
D.
To fix the sample
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Solution
The secondary antibody binds to the primary antibody and is often conjugated to a detectable marker to enhance signal detection.
Correct Answer:
A
— To bind to the primary antibody
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Q. What is the purpose of using a selectable marker in recombinant DNA technology?
A.
To enhance protein expression
B.
To identify successfully transformed cells
C.
To increase DNA stability
D.
To reduce mutation rates
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Solution
Selectable markers are used to identify successfully transformed cells that have taken up the recombinant DNA.
Correct Answer:
B
— To identify successfully transformed cells
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Q. What is the purpose of using deuterated solvents in NMR?
A.
To increase sample concentration
B.
To provide a clearer spectrum
C.
To reduce background noise
D.
To enhance signal strength
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Solution
Deuterated solvents are used to reduce background noise and provide clearer spectra by minimizing signals from the solvent itself.
Correct Answer:
B
— To provide a clearer spectrum
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Q. What is the purpose of using SDS-PAGE in protein analysis?
A.
To determine protein concentration
B.
To separate proteins based on size
C.
To identify protein sequences
D.
To purify proteins
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Solution
SDS-PAGE is used to separate proteins based on their size, allowing for analysis of protein purity and molecular weight.
Correct Answer:
B
— To separate proteins based on size
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Q. What is the recommended daily intake of carbohydrates for an average adult?
A.
20-30 grams
B.
45-65% of total daily calories
C.
100-150 grams
D.
200-300 grams
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Solution
The recommended daily intake of carbohydrates is 45-65% of total daily calories.
Correct Answer:
B
— 45-65% of total daily calories
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Q. What is the role of a detector in spectroscopy?
A.
To ionize the sample
B.
To separate the components
C.
To measure the intensity of light
D.
To prepare the sample
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Solution
The role of a detector in spectroscopy is to measure the intensity of light that is transmitted or emitted from the sample.
Correct Answer:
C
— To measure the intensity of light
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Q. What is the role of affinity chromatography in protein purification?
A.
To separate proteins based on size
B.
To separate proteins based on charge
C.
To isolate proteins that bind to specific ligands
D.
To denature proteins for analysis
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Solution
Affinity chromatography is used to isolate proteins that specifically bind to certain ligands, allowing for targeted purification.
Correct Answer:
C
— To isolate proteins that bind to specific ligands
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Q. What is the role of ATP in cellular thermodynamics?
A.
Storage of genetic information
B.
Energy currency of the cell
C.
Structural component of membranes
D.
Signal transduction
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Solution
ATP serves as the energy currency of the cell, providing energy for various biochemical processes.
Correct Answer:
B
— Energy currency of the cell
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Q. What is the role of ATP synthase in oxidative phosphorylation?
A.
To transport electrons
B.
To pump protons
C.
To synthesize ATP
D.
To oxidize NADH
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Solution
ATP synthase synthesizes ATP from ADP and inorganic phosphate using the proton gradient.
Correct Answer:
C
— To synthesize ATP
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Q. What is the role of B cells in relation to antigens?
A.
They produce antibodies that bind to antigens
B.
They destroy antigens directly
C.
They present antigens to T cells
D.
They produce cytokines
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Solution
B cells produce antibodies that specifically bind to antigens.
Correct Answer:
A
— They produce antibodies that bind to antigens
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Q. What is the role of buffers in biological systems?
A.
Increase pH
B.
Decrease pH
C.
Maintain stable pH
D.
Change pH rapidly
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Solution
Buffers help maintain a stable pH in biological systems by neutralizing acids and bases.
Correct Answer:
C
— Maintain stable pH
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Q. What is the role of calcium ions in cell signaling?
A.
They act as a primary messenger
B.
They serve as a second messenger
C.
They inhibit signaling pathways
D.
They are involved in protein synthesis
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Solution
Calcium ions serve as a second messenger in various signaling pathways, facilitating communication within the cell.
Correct Answer:
B
— They serve as a second messenger
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Q. What is the role of coenzyme A in fatty acid oxidation?
A.
Electron carrier
B.
Acyl group carrier
C.
Energy donor
D.
Regulator of metabolism
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Solution
Coenzyme A acts as an acyl group carrier, facilitating the transport of fatty acids into the mitochondria for oxidation.
Correct Answer:
B
— Acyl group carrier
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