Biochemistry

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Q. What is the primary purpose of the mobile phase in HPLC?
  • A. To provide a stationary phase
  • B. To dissolve the sample
  • C. To detect the analytes
  • D. To inject the sample
Q. What is the primary reactant in photosynthesis?
  • A. Glucose
  • B. Oxygen
  • C. Carbon Dioxide
  • D. Water
Q. What is the primary role of allosteric enzymes in metabolic pathways?
  • A. To catalyze irreversible reactions
  • B. To provide feedback inhibition
  • C. To enhance substrate binding
  • D. To stabilize the transition state
Q. What is the primary role of cholesterol in the cell membrane?
  • A. To provide energy
  • B. To stabilize membrane fluidity
  • C. To facilitate transport
  • D. To act as a receptor
Q. What is the primary role of hemoglobin in the blood?
  • A. Transporting nutrients
  • B. Regulating pH
  • C. Transporting oxygen
  • D. Clotting blood
Q. What is the primary role of proteins in nucleic acid interactions?
  • A. Catalyzing chemical reactions
  • B. Storing genetic information
  • C. Facilitating transcription and replication
  • D. Providing structural support
Q. What is the primary role of the plasma membrane?
  • A. Energy production
  • B. Regulating what enters and exits the cell
  • C. Protein synthesis
  • D. Storage of genetic information
Q. What is the primary site of amino acid metabolism in the body?
  • A. Liver
  • B. Kidney
  • C. Muscle
  • D. Intestine
Q. What is the primary site of ATP synthesis in eukaryotic cells?
  • A. Cytoplasm
  • B. Mitochondria
  • C. Nucleus
  • D. Endoplasmic Reticulum
Q. What is the primary source of electrons for the electron transport chain?
  • A. Glucose
  • B. NADH and FADH2
  • C. Oxygen
  • D. ATP
Q. What is the primary source of nitrogen for the synthesis of non-essential amino acids?
  • A. Dietary proteins
  • B. Ammonia
  • C. Urea
  • D. Nucleic acids
Q. What is the primary structure of a peptide?
  • A. The sequence of amino acids
  • B. The three-dimensional shape
  • C. The arrangement of beta sheets
  • D. The interaction with other peptides
Q. What is the primary structure of RNA composed of?
  • A. Amino acids
  • B. Nucleotides
  • C. Fatty acids
  • D. Monosaccharides
Q. What is the process of copying DNA called?
  • A. Transcription
  • B. Translation
  • C. Replication
  • D. Mutation
Q. What is the proton motive force?
  • A. The energy from ATP hydrolysis
  • B. The gradient of protons across the membrane
  • C. The flow of electrons through the chain
  • D. The consumption of oxygen
Q. What is the purpose of a DNA ladder in gel electrophoresis?
  • A. To visualize the gel
  • B. To provide a size reference for DNA fragments
  • C. To enhance the resolution of bands
  • D. To stain the DNA
Q. What is the purpose of staining the gel after electrophoresis?
  • A. To enhance the gel's structural integrity
  • B. To visualize the separated DNA or proteins
  • C. To increase the gel's conductivity
  • D. To prevent degradation of samples
Q. What is the purpose of using a secondary antibody in immunological assays?
  • A. To bind to the primary antibody
  • B. To enhance the immune response
  • C. To isolate specific cells
  • D. To fix the sample
Q. What is the purpose of using a selectable marker in recombinant DNA technology?
  • A. To enhance protein expression
  • B. To identify successfully transformed cells
  • C. To increase DNA stability
  • D. To reduce mutation rates
Q. What is the purpose of using deuterated solvents in NMR?
  • A. To increase sample concentration
  • B. To provide a clearer spectrum
  • C. To reduce background noise
  • D. To enhance signal strength
Q. What is the purpose of using SDS-PAGE in protein analysis?
  • A. To determine protein concentration
  • B. To separate proteins based on size
  • C. To identify protein sequences
  • D. To purify proteins
Q. What is the recommended daily intake of carbohydrates for an average adult?
  • A. 20-30 grams
  • B. 45-65% of total daily calories
  • C. 100-150 grams
  • D. 200-300 grams
Q. What is the role of a detector in spectroscopy?
  • A. To ionize the sample
  • B. To separate the components
  • C. To measure the intensity of light
  • D. To prepare the sample
Q. What is the role of affinity chromatography in protein purification?
  • A. To separate proteins based on size
  • B. To separate proteins based on charge
  • C. To isolate proteins that bind to specific ligands
  • D. To denature proteins for analysis
Q. What is the role of ATP in cellular thermodynamics?
  • A. Storage of genetic information
  • B. Energy currency of the cell
  • C. Structural component of membranes
  • D. Signal transduction
Q. What is the role of ATP synthase in oxidative phosphorylation?
  • A. To transport electrons
  • B. To pump protons
  • C. To synthesize ATP
  • D. To oxidize NADH
Q. What is the role of B cells in relation to antigens?
  • A. They produce antibodies that bind to antigens
  • B. They destroy antigens directly
  • C. They present antigens to T cells
  • D. They produce cytokines
Q. What is the role of buffers in biological systems?
  • A. Increase pH
  • B. Decrease pH
  • C. Maintain stable pH
  • D. Change pH rapidly
Q. What is the role of calcium ions in cell signaling?
  • A. They act as a primary messenger
  • B. They serve as a second messenger
  • C. They inhibit signaling pathways
  • D. They are involved in protein synthesis
Q. What is the role of coenzyme A in fatty acid oxidation?
  • A. Electron carrier
  • B. Acyl group carrier
  • C. Energy donor
  • D. Regulator of metabolism
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