Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. What is the primary factor that affects protein stability in a cellular environment?
A.
Temperature
B.
pH level
C.
Ionic strength
D.
All of the above
Show solution
Solution
All of the above factors can significantly influence protein stability.
Correct Answer:
D
— All of the above
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Q. What is the primary function of antibodies in the immune system?
A.
To transport oxygen in the blood
B.
To neutralize pathogens
C.
To produce hormones
D.
To regulate body temperature
Show solution
Solution
Antibodies are proteins produced by the immune system that specifically recognize and neutralize pathogens such as bacteria and viruses.
Correct Answer:
B
— To neutralize pathogens
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Q. What is the primary function of antibodies?
A.
To destroy pathogens directly
B.
To bind to antigens and neutralize them
C.
To produce more antigens
D.
To activate T cells
Show solution
Solution
The primary function of antibodies is to bind to antigens and neutralize them.
Correct Answer:
B
— To bind to antigens and neutralize them
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Q. What is the primary function of branched-chain amino acids (BCAAs)?
A.
Energy production
B.
Protein synthesis
C.
Hormone regulation
D.
Neurotransmitter synthesis
Show solution
Solution
Branched-chain amino acids (leucine, isoleucine, and valine) primarily promote protein synthesis and muscle recovery.
Correct Answer:
B
— Protein synthesis
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Q. What is the primary function of DNA in cells?
A.
Energy production
B.
Protein synthesis
C.
Genetic information storage
D.
Cellular respiration
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Solution
DNA primarily serves as the genetic blueprint for the development and functioning of living organisms.
Correct Answer:
C
— Genetic information storage
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Q. What is the primary function of enhancers in gene regulation?
A.
To terminate transcription
B.
To promote transcription from a distance
C.
To splice RNA
D.
To degrade mRNA
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Solution
Enhancers are regulatory DNA sequences that promote transcription from a distance by interacting with transcription factors.
Correct Answer:
B
— To promote transcription from a distance
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Q. What is the primary function of enzymes in the body?
A.
To provide energy
B.
To catalyze biochemical reactions
C.
To transport oxygen
D.
To store genetic information
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Solution
Enzymes act as catalysts that speed up biochemical reactions without being consumed in the process.
Correct Answer:
B
— To catalyze biochemical reactions
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Q. What is the primary function of G-protein coupled receptors (GPCRs) in cell signaling?
A.
Transport ions across the membrane
B.
Activate intracellular signaling pathways
C.
Synthesize proteins
D.
Degrade extracellular signals
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Solution
GPCRs primarily function to activate intracellular signaling pathways upon binding to ligands.
Correct Answer:
B
— Activate intracellular signaling pathways
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Q. What is the primary function of mRNA in protein synthesis?
A.
To transport amino acids
B.
To carry genetic information from DNA
C.
To form the ribosome structure
D.
To catalyze peptide bond formation
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Solution
mRNA carries genetic information from DNA to the ribosome for protein synthesis.
Correct Answer:
B
— To carry genetic information from DNA
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Q. What is the primary function of oxidative phosphorylation in cellular respiration?
A.
To produce glucose
B.
To generate ATP
C.
To consume oxygen
D.
To produce carbon dioxide
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Solution
Oxidative phosphorylation primarily generates ATP through the electron transport chain and chemiosmosis.
Correct Answer:
B
— To generate ATP
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Q. What is the primary function of T cells in the immune response?
A.
Produce antibodies
B.
Directly kill infected cells
C.
Activate B cells
D.
Phagocytize pathogens
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Solution
T cells are primarily responsible for directly killing infected cells and coordinating the immune response.
Correct Answer:
B
— Directly kill infected cells
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Q. What is the primary function of T cells in the immune system?
A.
Produce antibodies
B.
Destroy infected cells
C.
Activate B cells
D.
Phagocytize pathogens
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Solution
T cells are primarily responsible for directly killing infected cells and coordinating the immune response.
Correct Answer:
B
— Destroy infected cells
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Q. What is the primary function of the lac operon in E. coli?
A.
To regulate amino acid synthesis
B.
To control lactose metabolism
C.
To enhance DNA replication
D.
To promote cell division
Show solution
Solution
The lac operon regulates the metabolism of lactose in E. coli by controlling the expression of genes required for lactose uptake and breakdown.
Correct Answer:
B
— To control lactose metabolism
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Q. What is the primary function of the nucleus in a cell?
A.
Energy production
B.
Protein synthesis
C.
Genetic material storage
D.
Cellular respiration
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Solution
The nucleus stores the cell's genetic material (DNA) and is responsible for regulating gene expression.
Correct Answer:
C
— Genetic material storage
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Q. What is the primary function of the phospholipid bilayer in cell membranes?
A.
Energy production
B.
Selective permeability
C.
Protein synthesis
D.
Cell division
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Solution
The phospholipid bilayer provides selective permeability, allowing certain substances to pass while blocking others.
Correct Answer:
B
— Selective permeability
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Q. What is the primary function of the TCA cycle?
A.
Fatty acid synthesis
B.
Amino acid degradation
C.
Energy production through oxidation of acetyl-CoA
D.
Nucleotide synthesis
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Solution
The TCA cycle primarily functions in the oxidation of acetyl-CoA to produce energy in the form of ATP.
Correct Answer:
C
— Energy production through oxidation of acetyl-CoA
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Q. What is the primary function of thermodynamics in biological systems?
A.
To regulate blood pressure
B.
To determine energy transfer and transformation
C.
To control hormone levels
D.
To manage immune responses
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Solution
Thermodynamics helps in understanding how energy is transferred and transformed in biological systems.
Correct Answer:
B
— To determine energy transfer and transformation
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Q. What is the primary function of transcription factors?
A.
To degrade RNA
B.
To initiate transcription of specific genes
C.
To replicate DNA
D.
To package DNA
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Solution
Transcription factors are proteins that bind to specific DNA sequences to regulate the transcription of genes, initiating the process.
Correct Answer:
B
— To initiate transcription of specific genes
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Q. What is the primary function of Vitamin B1 (Thiamine)?
A.
Antioxidant
B.
Energy metabolism
C.
Bone health
D.
Vision
Show solution
Solution
Vitamin B1 (Thiamine) is crucial for energy metabolism, particularly in carbohydrate metabolism.
Correct Answer:
B
— Energy metabolism
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Q. What is the primary function of Vitamin B12?
A.
DNA synthesis
B.
Antioxidant
C.
Calcium absorption
D.
Collagen formation
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Solution
Vitamin B12 is essential for DNA synthesis and the formation of red blood cells.
Correct Answer:
A
— DNA synthesis
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Q. What is the primary immune-mediated condition that affects the gastrointestinal tract and is triggered by gluten?
A.
Celiac Disease
B.
Ulcerative Colitis
C.
Crohn's Disease
D.
Irritable Bowel Syndrome
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Solution
Celiac Disease is an autoimmune disorder where ingestion of gluten leads to damage in the small intestine.
Correct Answer:
A
— Celiac Disease
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Q. What is the primary organ responsible for nitrogen metabolism in the human body?
A.
Liver
B.
Kidney
C.
Lungs
D.
Spleen
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Solution
The liver is the primary organ responsible for nitrogen metabolism, including the urea cycle.
Correct Answer:
A
— Liver
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Q. What is the primary principle behind infrared spectroscopy?
A.
Absorption of UV light
B.
Vibrational transitions of molecules
C.
Emission of X-rays
D.
Scattering of visible light
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Solution
Infrared spectroscopy is based on the absorption of infrared light, which causes vibrational transitions in molecules.
Correct Answer:
B
— Vibrational transitions of molecules
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Q. What is the primary principle behind NMR spectroscopy?
A.
Ionization of molecules
B.
Magnetic properties of nuclei
C.
Absorption of UV light
D.
Fluorescence of compounds
Show solution
Solution
NMR spectroscopy is based on the magnetic properties of certain nuclei, which resonate in a magnetic field.
Correct Answer:
B
— Magnetic properties of nuclei
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Q. What is the primary purpose of chromatography in a clinical laboratory?
A.
To separate and analyze complex mixtures
B.
To sterilize medical equipment
C.
To measure blood pressure
D.
To perform surgical procedures
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Solution
Chromatography is primarily used to separate and analyze complex mixtures in clinical laboratories.
Correct Answer:
A
— To separate and analyze complex mixtures
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Q. What is the primary purpose of ELISA in immunology?
A.
To measure the concentration of antibodies
B.
To isolate specific cells
C.
To visualize tissue samples
D.
To amplify DNA
Show solution
Solution
ELISA (Enzyme-Linked Immunosorbent Assay) is primarily used to measure the concentration of antibodies or antigens in a sample.
Correct Answer:
A
— To measure the concentration of antibodies
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Q. What is the primary purpose of gas chromatography in clinical laboratories?
A.
To separate and analyze volatile compounds
B.
To measure blood glucose levels
C.
To perform DNA sequencing
D.
To culture bacteria
Show solution
Solution
Gas chromatography is primarily used to separate and analyze volatile compounds in various samples.
Correct Answer:
A
— To separate and analyze volatile compounds
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Q. What is the primary purpose of gel electrophoresis in molecular biology?
A.
To amplify DNA sequences
B.
To separate nucleic acids or proteins based on size
C.
To sequence DNA
D.
To clone genes
Show solution
Solution
Gel electrophoresis is primarily used to separate nucleic acids or proteins based on their size.
Correct Answer:
B
— To separate nucleic acids or proteins based on size
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Q. What is the primary purpose of protein purification?
A.
To increase protein concentration
B.
To isolate a specific protein from a mixture
C.
To denature proteins
D.
To enhance protein activity
Show solution
Solution
The primary purpose of protein purification is to isolate a specific protein from a mixture for further study or use.
Correct Answer:
B
— To isolate a specific protein from a mixture
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Q. What is the primary purpose of recombinant DNA technology?
A.
To create genetically modified organisms
B.
To clone human organs
C.
To sequence human genomes
D.
To diagnose genetic disorders
Show solution
Solution
Recombinant DNA technology is primarily used to create genetically modified organisms by combining DNA from different sources.
Correct Answer:
A
— To create genetically modified organisms
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