Biochemistry MCQ & Objective Questions
Biochemistry is a crucial subject for students aspiring to excel in their school exams and competitive tests. Understanding the biochemical processes that underpin life is essential for scoring well. Practicing MCQs and objective questions in Biochemistry not only enhances your grasp of the subject but also boosts your confidence during exams. Engaging with these practice questions helps in identifying important concepts and prepares you effectively for various assessments.
What You Will Practise Here
Fundamental concepts of biomolecules: carbohydrates, proteins, lipids, and nucleic acids.
Metabolic pathways: glycolysis, Krebs cycle, and oxidative phosphorylation.
Enzyme kinetics and regulation: understanding enzyme activity and factors affecting it.
Cellular respiration and photosynthesis: key processes in energy production.
Biochemical techniques: chromatography, electrophoresis, and spectrophotometry.
Genetic information flow: DNA replication, transcription, and translation.
Important biochemical equations and their applications in real-life scenarios.
Exam Relevance
Biochemistry is a significant part of the curriculum for CBSE, State Boards, NEET, and JEE. It often appears in the form of direct questions, application-based scenarios, and conceptual MCQs. Students can expect questions that assess their understanding of metabolic pathways, enzyme functions, and the role of biomolecules in living organisms. Familiarity with common question patterns, such as multiple-choice questions that require critical thinking, is essential for success in these exams.
Common Mistakes Students Make
Confusing the structures and functions of different biomolecules.
Misunderstanding metabolic pathways and their regulatory mechanisms.
Neglecting the importance of diagrams and flowcharts in explaining biochemical processes.
Overlooking the significance of enzyme kinetics in practical applications.
FAQs
Question: What are some effective ways to study Biochemistry for exams?Answer: Focus on understanding key concepts, practice MCQs regularly, and use diagrams to visualize processes.
Question: How can I improve my performance in Biochemistry MCQs?Answer: Regular practice of objective questions and reviewing common mistakes will enhance your exam readiness.
Start solving Biochemistry MCQs today to test your understanding and solidify your knowledge. Remember, consistent practice is the key to mastering this vital subject and achieving your academic goals!
Q. What is the function of histones in relation to DNA?
A.
To degrade RNA
B.
To package DNA into nucleosomes
C.
To synthesize proteins
D.
To repair DNA damage
Show solution
Solution
Histones are proteins that package and order DNA into structural units called nucleosomes, playing a key role in gene regulation.
Correct Answer:
B
— To package DNA into nucleosomes
Learn More →
Q. What is the function of membrane transport proteins?
A.
To synthesize lipids
B.
To facilitate the movement of substances across the membrane
C.
To provide structural support
D.
To store genetic information
Show solution
Solution
Membrane transport proteins assist in the movement of substances across the cell membrane, either passively or actively.
Correct Answer:
B
— To facilitate the movement of substances across the membrane
Learn More →
Q. What is the function of phagocytes in the immune system?
A.
Produce antibodies
B.
Engulf and digest pathogens
C.
Activate T cells
D.
Release cytokines
Show solution
Solution
Phagocytes, such as macrophages and neutrophils, engulf and digest pathogens as part of the immune response.
Correct Answer:
B
— Engulf and digest pathogens
Learn More →
Q. What is the function of phosphatases in cell signaling?
A.
To add phosphate groups to proteins
B.
To remove phosphate groups from proteins
C.
To transport signals across membranes
D.
To amplify the signal
Show solution
Solution
Phosphatases are enzymes that remove phosphate groups from proteins, thus playing a critical role in deactivating signaling pathways.
Correct Answer:
B
— To remove phosphate groups from proteins
Learn More →
Q. What is the function of primase in DNA replication?
A.
Unwinds DNA
B.
Synthesizes RNA primers
C.
Adds nucleotides
D.
Seals gaps
Show solution
Solution
Primase synthesizes short RNA primers needed for DNA polymerase to start replication.
Correct Answer:
B
— Synthesizes RNA primers
Learn More →
Q. What is the function of the complement system?
A.
Produce antibodies
B.
Enhance inflammation
C.
Lyse pathogens
D.
All of the above
Show solution
Solution
The complement system enhances inflammation, opsonizes pathogens, and can directly lyse them.
Correct Answer:
D
— All of the above
Learn More →
Q. What is the function of the endoplasmic reticulum?
A.
Energy production
B.
Synthesis of lipids and proteins
C.
Cell division
D.
DNA replication
Show solution
Solution
The endoplasmic reticulum is involved in the synthesis of lipids and proteins, with rough ER being studded with ribosomes for protein synthesis.
Correct Answer:
B
— Synthesis of lipids and proteins
Learn More →
Q. What is the main advantage of high-performance liquid chromatography (HPLC)?
A.
It requires larger sample sizes
B.
It provides faster separation and higher resolution
C.
It is less expensive than other methods
D.
It does not require a solvent
Show solution
Solution
HPLC provides faster separation and higher resolution compared to other chromatography methods.
Correct Answer:
B
— It provides faster separation and higher resolution
Learn More →
Q. What is the main advantage of using gas chromatography over liquid chromatography?
A.
Higher sensitivity for volatile compounds
B.
Lower cost
C.
Easier sample preparation
D.
Wider range of analytes
Show solution
Solution
Gas chromatography offers higher sensitivity for volatile compounds compared to liquid chromatography.
Correct Answer:
A
— Higher sensitivity for volatile compounds
Learn More →
Q. What is the main advantage of using high-performance liquid chromatography (HPLC) for protein purification?
A.
It is less expensive
B.
It provides high resolution and speed
C.
It requires less sample
D.
It is easier to set up
Show solution
Solution
HPLC provides high resolution and speed, making it a powerful technique for protein purification.
Correct Answer:
B
— It provides high resolution and speed
Learn More →
Q. What is the main advantage of using Raman spectroscopy?
A.
It requires no sample preparation
B.
It can analyze solid samples only
C.
It is less expensive than other methods
D.
It provides information on molecular mass
Show solution
Solution
One of the main advantages of Raman spectroscopy is that it typically requires no sample preparation, allowing for in situ analysis.
Correct Answer:
A
— It requires no sample preparation
Learn More →
Q. What is the main advantage of using recombinant antibodies?
A.
They can be produced in large quantities
B.
They are more stable than natural antibodies
C.
They can be engineered for specific functions
D.
All of the above
Show solution
Solution
Recombinant antibodies can be produced in large quantities, are often more stable, and can be engineered for specific functions.
Correct Answer:
D
— All of the above
Learn More →
Q. What is the main application of the complement fixation test?
A.
To detect the presence of specific antibodies
B.
To measure cell proliferation
C.
To isolate DNA
D.
To visualize cellular structures
Show solution
Solution
The complement fixation test is primarily used to detect the presence of specific antibodies in a patient's serum.
Correct Answer:
A
— To detect the presence of specific antibodies
Learn More →
Q. What is the main dietary source of omega-3 fatty acids?
A.
Red meat
B.
Fish
C.
Dairy products
D.
Vegetable oils
Show solution
Solution
Fish, particularly fatty fish like salmon, are the primary dietary source of omega-3 fatty acids.
Correct Answer:
B
— Fish
Learn More →
Q. What is the main disadvantage of using paper chromatography?
A.
It is time-consuming
B.
It has low resolution
C.
It requires expensive equipment
D.
It cannot separate small molecules
Show solution
Solution
The main disadvantage of paper chromatography is its low resolution compared to other methods.
Correct Answer:
B
— It has low resolution
Learn More →
Q. What is the main energy currency of the cell?
A.
NADH
B.
FADH2
C.
ATP
D.
GTP
Show solution
Solution
ATP (adenosine triphosphate) is the main energy currency of the cell, used for various cellular processes.
Correct Answer:
C
— ATP
Learn More →
Q. What is the main function of flow cytometry?
A.
To analyze the physical and chemical characteristics of cells
B.
To culture bacteria
C.
To perform genetic sequencing
D.
To visualize cellular structures
Show solution
Solution
Flow cytometry is primarily used to analyze the physical and chemical characteristics of cells in a fluid as they pass through a laser.
Correct Answer:
A
— To analyze the physical and chemical characteristics of cells
Learn More →
Q. What is the main function of lipids in biological membranes?
A.
Energy storage
B.
Structural support
C.
Catalysis of reactions
D.
Information storage
Show solution
Solution
Lipids, particularly phospholipids, provide structural support and form the bilayer of biological membranes.
Correct Answer:
B
— Structural support
Learn More →
Q. What is the main purpose of cellular respiration?
A.
To produce glucose
B.
To convert glucose into ATP
C.
To absorb sunlight
D.
To release oxygen
Show solution
Solution
The main purpose of cellular respiration is to convert glucose into ATP, which is usable energy for cells.
Correct Answer:
B
— To convert glucose into ATP
Learn More →
Q. What is the main storage form of carbohydrates in the human body?
A.
Glucose
B.
Fructose
C.
Glycogen
D.
Sucrose
Show solution
Solution
Glycogen is the main storage form of carbohydrates in the body.
Correct Answer:
C
— Glycogen
Learn More →
Q. What is the net ATP yield from one molecule of glucose during glycolysis?
A.
2 ATP
B.
4 ATP
C.
6 ATP
D.
0 ATP
Show solution
Solution
The net yield of ATP from glycolysis is 2 ATP per molecule of glucose.
Correct Answer:
A
— 2 ATP
Learn More →
Q. What is the pH of human blood?
A.
6.8-7.2
B.
7.35-7.45
C.
7.5-7.8
D.
8.0-8.5
Show solution
Solution
The normal pH range of human blood is 7.35 to 7.45.
Correct Answer:
B
— 7.35-7.45
Learn More →
Q. What is the pH range of pure water at 25°C?
A.
0-3
B.
4-6
C.
7
D.
8-10
Show solution
Solution
Pure water has a neutral pH of 7 at 25°C.
Correct Answer:
C
— 7
Learn More →
Q. What is the pKa of the carboxyl group in amino acids?
A.
Around 2
B.
Around 7
C.
Around 9
D.
Around 12
Show solution
Solution
The pKa of the carboxyl group in amino acids is typically around 2, indicating its acidic nature.
Correct Answer:
A
— Around 2
Learn More →
Q. What is the primary application of UV luminance spectroscopy in clinical settings?
A.
Detecting bacterial infections
B.
Measuring blood glucose levels
C.
Analyzing protein structures
D.
Assessing skin damage from UV exposure
Show solution
Solution
UV luminance spectroscopy is primarily used to assess skin damage from UV exposure, helping to evaluate the risk of skin cancer.
Correct Answer:
D
— Assessing skin damage from UV exposure
Learn More →
Q. What is the primary component of a triglyceride?
A.
Glycerol and three fatty acids
B.
Phospholipids
C.
Cholesterol
D.
Amino acids
Show solution
Solution
A triglyceride is composed of glycerol and three fatty acids.
Correct Answer:
A
— Glycerol and three fatty acids
Learn More →
Q. What is the primary consequence of protein denaturation?
A.
Loss of function
B.
Increased solubility
C.
Enhanced stability
D.
No change in activity
Show solution
Solution
Denaturation typically results in a loss of protein function.
Correct Answer:
A
— Loss of function
Learn More →
Q. What is the primary effect of insulin binding to its receptor?
A.
Increased glucose uptake
B.
Decreased protein synthesis
C.
Inhibition of cell growth
D.
Increased lipid breakdown
Show solution
Solution
Insulin binding to its receptor primarily leads to increased glucose uptake by cells.
Correct Answer:
A
— Increased glucose uptake
Learn More →
Q. What is the primary end product of glycolysis?
A.
Lactate
B.
Pyruvate
C.
Acetyl-CoA
D.
Glucose
Show solution
Solution
The primary end product of glycolysis is pyruvate, which is formed from glucose.
Correct Answer:
B
— Pyruvate
Learn More →
Q. What is the primary enzyme responsible for synthesizing RNA during transcription?
A.
DNA polymerase
B.
RNA polymerase
C.
Ligase
D.
Helicase
Show solution
Solution
RNA polymerase is the enzyme that synthesizes RNA from a DNA template during transcription.
Correct Answer:
B
— RNA polymerase
Learn More →
Showing 61 to 90 of 412 (14 Pages)