Analytical Techniques

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The Analytical Techniques section focuses on the methods and instruments used for qualitative and quantitative chemical analysis, essential for understanding experimental chemistry, material characterization, and laboratory diagnostics. This module is designed for school (Class 11–12) and undergraduate students, with strong emphasis on conceptual clarity, instrumentation basics, and exam relevance.

In this section, you will study:

  • Classical analytical methods – qualitative analysis, gravimetric and volumetric analysis

  • Spectroscopic techniques – UV–Visible spectroscopy, IR spectroscopy, NMR (introductory level), and mass spectrometry

  • Chromatographic methods – paper chromatography, TLC, column chromatography, gas chromatography, and HPLC (basics)

  • Electroanalytical techniques – conductometry, potentiometry, pH measurement, and electrochemical cells

  • Thermal and surface analysis (introductory) – TGA, DTA, and DSC (UG level)

  • Instrumentation principles – working, components, and applications

  • Data interpretation – spectra analysis, peak identification, and quantitative calculations

  • NCERT- and UG-aligned explanations, supported by diagrams, numericals, MCQs, assertion–reason questions, and PYQs

The content is structured to bridge theory with laboratory practice, strengthen analytical and interpretation skills, and help students confidently answer instrumentation- and application-based questions in school and undergraduate examinations.

Develop a systematic and practical understanding of Analytical Techniques to master this crucial area of modern Chemistry.

Q. In UV-Vis spectroscopy, what does a higher absorbance indicate?
  • A. Lower concentration of the analyte
  • B. Higher concentration of the analyte
  • C. Higher wavelength of light
  • D. Lower energy of light
Q. In UV-Vis spectroscopy, what does a higher absorbance value indicate?
  • A. Lower concentration of the analyte
  • B. Higher concentration of the analyte
  • C. Higher wavelength of light
  • D. Lower path length of the sample
Q. In UV-Vis spectroscopy, what does a higher molar absorptivity indicate?
  • A. Lower concentration
  • B. Higher concentration
  • C. More efficient light absorption
  • D. Less efficient light absorption
Q. In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
  • A. Presence of proteins
  • B. Presence of nucleic acids
  • C. Presence of carbohydrates
  • D. Presence of lipids
Q. In UV-Vis spectroscopy, what does a peak at 280 nm typically indicate?
  • A. Presence of nucleic acids
  • B. Presence of proteins
  • C. Presence of carbohydrates
  • D. Presence of lipids
Q. In UV-Vis spectroscopy, what does a peak in the absorbance spectrum indicate?
  • A. The presence of a specific ion
  • B. The concentration of a gas
  • C. The molecular weight of a compound
  • D. The boiling point of a solution
Q. In UV-Vis spectroscopy, what does a peak in the absorption spectrum indicate?
  • A. The presence of impurities
  • B. The concentration of the sample
  • C. A specific electronic transition
  • D. The temperature of the sample
Q. In UV-Vis spectroscopy, what does a shift in the absorption peak indicate?
  • A. Change in concentration
  • B. Change in pH
  • C. Change in molecular structure
  • D. Change in temperature
Q. In UV-Vis spectroscopy, what does the Beer-Lambert law relate?
  • A. Wavelength and energy
  • B. Absorbance and concentration
  • C. Frequency and intensity
  • D. Temperature and pressure
Q. In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
  • A. Decreases absorbance
  • B. Increases absorbance
  • C. No effect on absorbance
  • D. Increases transmittance
Q. What does a broad peak in an IR spectrum typically indicate?
  • A. Strong hydrogen bonding
  • B. Weak molecular interactions
  • C. High molecular weight
  • D. Low concentration
Q. What does a positive test for sulfate ions typically produce when barium chloride is added?
  • A. A blue solution
  • B. A white precipitate
  • C. A yellow solution
  • D. No change
Q. What does the peak position in an IR spectrum indicate?
  • A. Concentration of the sample
  • B. Molecular weight of the compound
  • C. Type of functional groups present
  • D. Purity of the sample
Q. What does the term 'Beer-Lambert Law' describe?
  • A. The relationship between temperature and absorbance
  • B. The relationship between concentration and absorbance
  • C. The relationship between wavelength and absorbance
  • D. The relationship between time and absorbance
Q. What does the term 'chromophore' refer to in UV-Vis spectroscopy?
  • A. A molecule that fluoresces
  • B. A part of a molecule responsible for its color
  • C. A solvent used in the analysis
  • D. A detector used in the instrument
Q. What does the term 'retention time' refer to in chromatography?
  • A. The time taken for a sample to be injected
  • B. The time taken for a component to pass through the detector
  • C. The time a component spends in the stationary phase
  • D. The total time of the chromatographic run
Q. What indicator is commonly used in a strong acid-strong base titration?
  • A. Phenolphthalein
  • B. Methyl orange
  • C. Bromothymol blue
  • D. Litmus
Q. What is a back titration?
  • A. Titration of a solution with a known concentration
  • B. Titration where the endpoint is determined by color change
  • C. Titration of an excess reagent followed by titration of the excess
  • D. Titration that uses a solid reagent
Q. What is a common application of complexometric titration?
  • A. Determining the concentration of metals in solution
  • B. Measuring the acidity of a solution
  • C. Analyzing the purity of organic compounds
  • D. Identifying unknown substances
Q. What is a common application of IR spectroscopy in environmental analysis?
  • A. Measuring pH levels
  • B. Identifying pollutants
  • C. Determining molecular weight
  • D. Separating mixtures
Q. What is a common application of titration in the food industry?
  • A. Measuring the viscosity of oils
  • B. Determining the acidity of fruit juices
  • C. Analyzing the color of beverages
  • D. Separating food additives
Q. What is a common mistake made during titration?
  • A. Using a burette for titrant delivery
  • B. Not swirling the flask during titration
  • C. Using a pH meter for endpoint detection
  • D. Adding titrant too slowly
Q. What is a common mistake to avoid during a titration?
  • A. Using a burette for accurate measurement
  • B. Adding titrant too quickly
  • C. Using a proper indicator
  • D. Recording the final volume accurately
Q. What is a common mistake to avoid during titration?
  • A. Adding titrant too quickly
  • B. Using a clean burette
  • C. Measuring the volume accurately
  • D. Using the correct indicator
Q. What is a common mistake when performing a titration?
  • A. Not swirling the flask
  • B. Using too much titrant
  • C. Not recording the initial volume
  • D. All of the above
Q. What is a common use of UV-Vis spectroscopy in environmental analysis?
  • A. Measuring soil pH
  • B. Detecting heavy metals
  • C. Analyzing water quality
  • D. Identifying microorganisms
Q. What is a primary standard in titration?
  • A. A solution of known concentration
  • B. A pure substance used to prepare a solution
  • C. An indicator used in titration
  • D. A method of measuring pH
Q. What is a typical application of UV-Vis spectroscopy in food analysis?
  • A. Measuring pH
  • B. Determining fat content
  • C. Analyzing color and pigments
  • D. Identifying microbial contamination
Q. What is the Beer-Lambert Law equation?
  • A. A = εcl
  • B. A = c/εl
  • C. A = l/εc
  • D. A = cl/ε
Q. What is the Beer-Lambert Law used for in UV-Vis spectroscopy?
  • A. To calculate the wavelength of light
  • B. To determine the concentration of a solution
  • C. To measure the intensity of emitted light
  • D. To analyze the molecular structure
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