Q. What is the significance of methicillin-resistant Staphylococcus aureus (MRSA)?
-
A.
It is more virulent
-
B.
It is resistant to multiple antibiotics
-
C.
It causes more skin infections
-
D.
It is easier to treat
Solution
MRSA is significant because it is resistant to multiple antibiotics, making it harder to treat.
Correct Answer:
B
— It is resistant to multiple antibiotics
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Q. What is the significance of passaging in cell culture?
-
A.
To increase the number of cells
-
B.
To decrease the cell density
-
C.
To change the medium
-
D.
To freeze the cells
Solution
Passaging is the process of transferring cells to new culture vessels to increase the number of cells and prevent overcrowding.
Correct Answer:
A
— To increase the number of cells
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Q. What is the significance of pH in plant tissue culture media?
-
A.
Affects nutrient availability
-
B.
Influences color of the medium
-
C.
Determines the solidifying agent
-
D.
Regulates temperature
Solution
The pH of plant tissue culture media affects nutrient availability and overall cell growth.
Correct Answer:
A
— Affects nutrient availability
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Q. What is the significance of plasmids in bacterial gene transfer?
-
A.
They are essential for binary fission
-
B.
They can carry antibiotic resistance genes
-
C.
They are only found in pathogenic bacteria
-
D.
They do not participate in gene transfer
Solution
Plasmids can carry antibiotic resistance genes and are important in horizontal gene transfer.
Correct Answer:
B
— They can carry antibiotic resistance genes
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Q. What is the significance of soil pH in microbiology?
-
A.
It affects microbial diversity
-
B.
It determines soil texture
-
C.
It influences water retention
-
D.
It has no effect on microbes
Solution
Soil pH significantly affects microbial diversity and activity.
Correct Answer:
A
— It affects microbial diversity
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Q. What is the significance of structure-activity relationship (SAR) in drug synthesis?
-
A.
It helps in predicting drug interactions
-
B.
It aids in optimizing drug potency
-
C.
It determines the drug's market price
-
D.
It is irrelevant to drug design
Solution
SAR helps in optimizing the potency and efficacy of therapeutic agents.
Correct Answer:
B
— It aids in optimizing drug potency
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Q. What is the significance of the 'gene of interest' in recombinant DNA technology?
-
A.
It is the DNA sequence that is cloned
-
B.
It is the vector used for cloning
-
C.
It is the enzyme used for cutting DNA
-
D.
It is the host organism
Solution
The 'gene of interest' is the specific DNA sequence that researchers aim to clone and manipulate in recombinant DNA technology.
Correct Answer:
A
— It is the DNA sequence that is cloned
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Q. What is the significance of the 'maximum specific growth rate' in fermentation?
-
A.
Indicates the fastest growth rate
-
B.
Determines substrate concentration
-
C.
Measures product yield
-
D.
Indicates fermentation duration
Solution
The maximum specific growth rate indicates the fastest growth rate of microorganisms during fermentation under optimal conditions.
Correct Answer:
A
— Indicates the fastest growth rate
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Q. What is the significance of the 'passage number' in cell culture?
-
A.
It indicates the age of the cells
-
B.
It refers to the number of times cells have been subcultured
-
C.
It measures cell viability
-
D.
It determines the growth rate of the cells
Solution
The passage number indicates how many times the cells have been subcultured, which is important for maintaining cell characteristics.
Correct Answer:
B
— It refers to the number of times cells have been subcultured
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Q. What is the significance of the 'tail' structure in bacteriophages?
-
A.
It helps in replication
-
B.
It aids in attachment to host bacteria
-
C.
It protects the genetic material
-
D.
It is used for energy production
Solution
The tail structure of bacteriophages is crucial for attaching to and injecting their genetic material into host bacteria.
Correct Answer:
B
— It aids in attachment to host bacteria
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Q. What is the significance of the affinity of an antibody for its antigen?
-
A.
It determines the speed of the immune response
-
B.
It affects the strength of the binding interaction
-
C.
It influences the type of immune cells activated
-
D.
It has no clinical significance
Solution
The affinity of an antibody for its antigen affects the strength of the binding interaction, which is crucial for effective immune response.
Correct Answer:
B
— It affects the strength of the binding interaction
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Q. What is the significance of the annealing temperature in PCR?
-
A.
It determines the speed of DNA synthesis
-
B.
It affects the specificity of primer binding
-
C.
It controls the amount of DNA produced
-
D.
It is irrelevant to the PCR process
Solution
The annealing temperature is crucial as it affects the specificity of primer binding to the target DNA.
Correct Answer:
B
— It affects the specificity of primer binding
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Q. What is the significance of the AUG codon in translation?
-
A.
It signals the end of translation
-
B.
It codes for the amino acid methionine and initiates translation
-
C.
It is a stop codon
-
D.
It is involved in mRNA splicing
Solution
The AUG codon codes for methionine and serves as the start codon for translation.
Correct Answer:
B
— It codes for the amino acid methionine and initiates translation
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Q. What is the significance of the CRISPR-Cas9 technology in plant genetics?
-
A.
It allows for the cloning of plants
-
B.
It enables precise gene editing
-
C.
It increases plant growth rate
-
D.
It enhances photosynthetic efficiency
Solution
CRISPR-Cas9 is a revolutionary tool that allows for precise editing of plant genomes.
Correct Answer:
B
— It enables precise gene editing
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Q. What is the significance of the equilibrium constant (K) in thermodynamics?
-
A.
It indicates the speed of a reaction
-
B.
It determines the direction of a reaction
-
C.
It reflects the ratio of products to reactants at equilibrium
-
D.
It measures the energy change of a reaction
Solution
The equilibrium constant (K) reflects the ratio of products to reactants at equilibrium, indicating the extent of the reaction.
Correct Answer:
C
— It reflects the ratio of products to reactants at equilibrium
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Q. What is the significance of the lac operon in E. coli?
-
A.
It regulates amino acid synthesis
-
B.
It controls lactose metabolism
-
C.
It is involved in DNA repair
-
D.
It facilitates cell division
Solution
The lac operon is a set of genes that regulates the metabolism of lactose in E. coli, allowing it to utilize lactose as an energy source.
Correct Answer:
B
— It controls lactose metabolism
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Q. What is the significance of the nitrogen cycle in ecosystems?
-
A.
It helps in energy transfer
-
B.
It recycles nutrients
-
C.
It increases carbon dioxide levels
-
D.
It reduces soil acidity
Solution
The nitrogen cycle is crucial for recycling nitrogen in various forms, making it available for plant use.
Correct Answer:
B
— It recycles nutrients
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Q. What is the significance of the polymerase chain reaction (PCR) in recombinant DNA technology?
-
A.
It cuts DNA into fragments
-
B.
It amplifies specific DNA sequences
-
C.
It joins DNA fragments
-
D.
It sequences DNA
Solution
PCR is significant because it amplifies specific DNA sequences, making it easier to work with small amounts of DNA.
Correct Answer:
B
— It amplifies specific DNA sequences
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Q. What is the significance of the protein p53 in relation to DNA?
-
A.
It promotes DNA replication
-
B.
It acts as a tumor suppressor by regulating the cell cycle
-
C.
It synthesizes RNA
-
D.
It repairs DNA damage
Solution
p53 is a crucial tumor suppressor protein that regulates the cell cycle and helps prevent the proliferation of cells with damaged DNA.
Correct Answer:
B
— It acts as a tumor suppressor by regulating the cell cycle
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Q. What is the significance of the start codon?
-
A.
It signals the end of translation
-
B.
It codes for a stop signal
-
C.
It initiates protein synthesis
-
D.
It regulates gene expression
Solution
The start codon (AUG) signals the beginning of translation and codes for the amino acid methionine.
Correct Answer:
C
— It initiates protein synthesis
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Q. What is the significance of tumor grading?
-
A.
Determines the tumor's size
-
B.
Predicts the tumor's behavior and prognosis
-
C.
Identifies the tumor's location
-
D.
Measures the patient's response to treatment
Solution
Tumor grading is significant as it predicts the tumor's behavior and prognosis based on the degree of differentiation and histological features.
Correct Answer:
B
— Predicts the tumor's behavior and prognosis
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Q. What is the significance of using a buffer during protein purification?
-
A.
To increase protein solubility
-
B.
To maintain pH and ionic strength
-
C.
To enhance protein activity
-
D.
To precipitate unwanted proteins
Solution
Buffers are used during protein purification to maintain pH and ionic strength, which are critical for protein stability and solubility.
Correct Answer:
B
— To maintain pH and ionic strength
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Q. What is the significance of viral load in HIV treatment?
-
A.
Indicates the presence of antibodies
-
B.
Measures the number of viral particles in the blood
-
C.
Determines the immune response
-
D.
Indicates the effectiveness of antibiotics
Solution
Viral load measures the number of viral particles in the blood, which is crucial for monitoring HIV treatment efficacy.
Correct Answer:
B
— Measures the number of viral particles in the blood
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Q. What is the structure of an antibody?
-
A.
Single polypeptide chain
-
B.
Two heavy and two light chains
-
C.
Three heavy chains
-
D.
Four light chains
Solution
Antibodies are composed of two heavy chains and two light chains, forming a Y-shaped structure that allows for antigen binding.
Correct Answer:
B
— Two heavy and two light chains
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Q. What is the structure of DNA commonly described as?
-
A.
Single helix
-
B.
Double helix
-
C.
Triple helix
-
D.
Linear strand
Solution
DNA is commonly described as a double helix, consisting of two strands wound around each other.
Correct Answer:
B
— Double helix
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Q. What is the sugar component of RNA?
-
A.
Deoxyribose
-
B.
Ribose
-
C.
Glucose
-
D.
Fructose
Solution
The sugar component of RNA is ribose, which differs from deoxyribose found in DNA.
Correct Answer:
B
— Ribose
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Q. What is the term for animals that have had their genome altered by the transfer of a gene from another species?
-
A.
Cloned animals
-
B.
Transgenic animals
-
C.
Hybrid animals
-
D.
Genetically modified organisms
Solution
Transgenic animals are those that have had their genome altered by the transfer of a gene from another species.
Correct Answer:
B
— Transgenic animals
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Q. What is the term for bacteria that can grow in the absence of oxygen?
-
A.
Aerobes
-
B.
Anaerobes
-
C.
Facultative anaerobes
-
D.
Microaerophiles
Solution
Anaerobes are bacteria that can grow in the absence of oxygen.
Correct Answer:
B
— Anaerobes
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Q. What is the term for bacteria that thrive in high salt concentrations?
-
A.
Mesophiles
-
B.
Halophiles
-
C.
Psychrophiles
-
D.
Thermophiles
Solution
Halophiles are bacteria that thrive in high salt concentrations.
Correct Answer:
B
— Halophiles
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Q. What is the term for the ability of antibodies to bind specifically to antigens?
-
A.
Affinity
-
B.
Specificity
-
C.
Cross-reactivity
-
D.
Neutralization
Solution
Affinity refers to the strength of the interaction between an antibody and its specific antigen.
Correct Answer:
B
— Specificity
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