Q. What is the purpose of using SDS-PAGE in protein analysis?
-
A.
To determine protein concentration
-
B.
To separate proteins based on size
-
C.
To identify protein sequences
-
D.
To purify proteins
Solution
SDS-PAGE is used to separate proteins based on their size, allowing for analysis of protein purity and molecular weight.
Correct Answer:
B
— To separate proteins based on size
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Q. What is the recommended agitation technique for mixing blood products?
-
A.
Vigorous shaking
-
B.
Gentle inversion
-
C.
Rapid spinning
-
D.
Continuous stirring
Solution
Gentle inversion is the recommended technique to mix blood products without causing damage.
Correct Answer:
B
— Gentle inversion
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Q. What is the recommended daily intake of carbohydrates for an average adult?
-
A.
20-30 grams
-
B.
45-65% of total daily calories
-
C.
100-150 grams
-
D.
200-300 grams
Solution
The recommended daily intake of carbohydrates is 45-65% of total daily calories.
Correct Answer:
B
— 45-65% of total daily calories
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Q. What is the recommended internal temperature for cooked chicken?
-
A.
145°F
-
B.
160°F
-
C.
165°F
-
D.
170°F
Solution
The recommended internal temperature for cooked chicken is 165°F to ensure safety.
Correct Answer:
C
— 165°F
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Q. What is the recommended internal temperature for safely heating canned foods?
-
A.
60°C (140°F)
-
B.
75°C (167°F)
-
C.
85°C (185°F)
-
D.
100°C (212°F)
Solution
Canned foods should be heated to an internal temperature of at least 75°C (167°F) to ensure safety.
Correct Answer:
B
— 75°C (167°F)
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Q. What is the recommended maximum consumption of fish per week to minimize mercury exposure?
-
A.
1 serving
-
B.
2-3 servings
-
C.
4-5 servings
-
D.
6-7 servings
Solution
It is generally recommended to limit fish consumption to 2-3 servings per week to minimize mercury exposure.
Correct Answer:
B
— 2-3 servings
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Q. What is the recommended moderate alcohol consumption limit for women per day?
-
A.
1 drink
-
B.
2 drinks
-
C.
3 drinks
-
D.
4 drinks
Solution
The recommended moderate alcohol consumption limit for women is 1 drink per day.
Correct Answer:
A
— 1 drink
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Q. What is the result of transcription?
-
A.
DNA replication
-
B.
Protein synthesis
-
C.
RNA synthesis
-
D.
Cell division
Solution
The result of transcription is the synthesis of RNA from a DNA template.
Correct Answer:
C
— RNA synthesis
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Q. What is the role of a bioreactor's agitator?
-
A.
To cool the reactor
-
B.
To mix the contents
-
C.
To sterilize the medium
-
D.
To measure pH
Solution
The agitator's role is to mix the contents of the bioreactor, ensuring uniform distribution of nutrients and microorganisms.
Correct Answer:
B
— To mix the contents
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Q. What is the role of a carrier in enzyme immobilization?
-
A.
To increase enzyme activity
-
B.
To provide a surface for enzyme attachment
-
C.
To decrease reaction time
-
D.
To enhance substrate binding
Solution
The role of a carrier in enzyme immobilization is to provide a surface for enzyme attachment, facilitating the immobilization process.
Correct Answer:
B
— To provide a surface for enzyme attachment
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Q. What is the role of a coenzyme in enzyme activity?
-
A.
To provide structural support
-
B.
To act as a substrate
-
C.
To assist in enzyme-substrate binding
-
D.
To participate in the reaction as a transient carrier
Solution
Coenzymes are organic molecules that assist enzymes by carrying chemical groups during reactions.
Correct Answer:
D
— To participate in the reaction as a transient carrier
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Q. What is the role of a detector in spectroscopy?
-
A.
To ionize the sample
-
B.
To separate the components
-
C.
To measure the intensity of light
-
D.
To prepare the sample
Solution
The role of a detector in spectroscopy is to measure the intensity of light that is transmitted or emitted from the sample.
Correct Answer:
C
— To measure the intensity of light
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Q. What is the role of a fermenter in biochemical engineering?
-
A.
To separate products from cells
-
B.
To provide a controlled environment for fermentation
-
C.
To analyze metabolic pathways
-
D.
To synthesize DNA
Solution
A fermenter provides a controlled environment for fermentation, allowing microorganisms to convert substrates into desired products.
Correct Answer:
B
— To provide a controlled environment for fermentation
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Q. What is the role of a promoter in a cloning vector?
-
A.
To initiate transcription
-
B.
To terminate transcription
-
C.
To replicate DNA
-
D.
To bind ribosomes
Solution
A promoter in a cloning vector is essential for initiating the transcription of the inserted gene.
Correct Answer:
A
— To initiate transcription
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Q. What is the role of a public health surveillance system?
-
A.
To provide direct medical care to patients
-
B.
To monitor and analyze health data to inform public health actions
-
C.
To conduct laboratory research
-
D.
To develop new pharmaceuticals
Solution
Public health surveillance systems collect and analyze health data to guide public health policy and practice.
Correct Answer:
B
— To monitor and analyze health data to inform public health actions
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Q. What is the role of a selectable marker in a vector?
-
A.
To enhance cell adhesion
-
B.
To allow for the selection of successfully transfected cells
-
C.
To increase protein expression
-
D.
To provide energy to the cells
Solution
Selectable markers are used to identify and select cells that have successfully incorporated the vector.
Correct Answer:
B
— To allow for the selection of successfully transfected cells
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Q. What is the role of activated carbon in water treatment?
-
A.
To add minerals
-
B.
To remove chlorine
-
C.
To adsorb organic compounds
-
D.
To increase pH
Solution
Activated carbon is used to adsorb organic compounds and improve water quality.
Correct Answer:
C
— To adsorb organic compounds
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Q. What is the role of affinity chromatography in protein purification?
-
A.
To separate proteins based on size
-
B.
To separate proteins based on charge
-
C.
To isolate proteins that bind to specific ligands
-
D.
To denature proteins for analysis
Solution
Affinity chromatography is used to isolate proteins that specifically bind to certain ligands, allowing for targeted purification.
Correct Answer:
C
— To isolate proteins that bind to specific ligands
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Q. What is the role of aldosterone in renal physiology?
-
A.
Increases sodium reabsorption
-
B.
Decreases potassium secretion
-
C.
Inhibits water reabsorption
-
D.
Stimulates urine production
Solution
Aldosterone increases sodium reabsorption in the kidneys, which helps to regulate blood pressure and fluid balance.
Correct Answer:
A
— Increases sodium reabsorption
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Q. What is the role of allosteric sites in enzyme regulation?
-
A.
To bind substrates
-
B.
To bind inhibitors only
-
C.
To bind activators or inhibitors that change enzyme shape
-
D.
To increase the temperature of the reaction
Solution
Allosteric sites are regions on enzymes where molecules can bind, causing a change in the enzyme's shape and activity, thus regulating its function.
Correct Answer:
C
— To bind activators or inhibitors that change enzyme shape
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Q. What is the role of anaerobic bacteria in wastewater treatment?
-
A.
To oxidize organic matter
-
B.
To produce methane
-
C.
To filter solids
-
D.
To remove heavy metals
Solution
Anaerobic bacteria play a crucial role in wastewater treatment by breaking down organic matter and producing methane in the absence of oxygen.
Correct Answer:
B
— To produce methane
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Q. What is the role of ATP in cellular thermodynamics?
-
A.
Storage of genetic information
-
B.
Energy currency of the cell
-
C.
Structural component of membranes
-
D.
Signal transduction
Solution
ATP serves as the energy currency of the cell, providing energy for various biochemical processes.
Correct Answer:
B
— Energy currency of the cell
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Q. What is the role of ATP synthase in oxidative phosphorylation?
-
A.
To transport electrons
-
B.
To pump protons
-
C.
To synthesize ATP
-
D.
To oxidize NADH
Solution
ATP synthase synthesizes ATP from ADP and inorganic phosphate using the proton gradient.
Correct Answer:
C
— To synthesize ATP
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Q. What is the role of auxins in plant cell culture?
-
A.
Promote root development
-
B.
Inhibit cell division
-
C.
Enhance photosynthesis
-
D.
Increase flowering
Solution
Auxins are plant hormones that primarily promote root development in plant cell culture.
Correct Answer:
A
— Promote root development
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Q. What is the role of B cells in relation to antigens?
-
A.
They produce antibodies that bind to antigens
-
B.
They destroy antigens directly
-
C.
They present antigens to T cells
-
D.
They produce cytokines
Solution
B cells produce antibodies that specifically bind to antigens.
Correct Answer:
A
— They produce antibodies that bind to antigens
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Q. What is the role of baroreceptors in the cardiovascular system?
-
A.
To detect changes in blood glucose levels
-
B.
To sense changes in blood pressure
-
C.
To monitor oxygen levels in the blood
-
D.
To regulate body temperature
Solution
Baroreceptors are specialized sensors that detect changes in blood pressure and help regulate cardiovascular function.
Correct Answer:
B
— To sense changes in blood pressure
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Q. What is the role of buffers in biological systems?
-
A.
Increase pH
-
B.
Decrease pH
-
C.
Maintain stable pH
-
D.
Change pH rapidly
Solution
Buffers help maintain a stable pH in biological systems by neutralizing acids and bases.
Correct Answer:
C
— Maintain stable pH
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Q. What is the role of calcium ions in cell signaling?
-
A.
They act as a primary messenger
-
B.
They serve as a second messenger
-
C.
They inhibit signaling pathways
-
D.
They are involved in protein synthesis
Solution
Calcium ions serve as a second messenger in various signaling pathways, facilitating communication within the cell.
Correct Answer:
B
— They serve as a second messenger
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Q. What is the role of chromatography in downstream processing?
-
A.
To kill microorganisms
-
B.
To concentrate the product
-
C.
To separate and purify biomolecules
-
D.
To enhance product stability
Solution
Chromatography is used to separate and purify biomolecules based on their properties.
Correct Answer:
C
— To separate and purify biomolecules
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Q. What is the role of coenzyme A in fatty acid oxidation?
-
A.
Electron carrier
-
B.
Acyl group carrier
-
C.
Energy donor
-
D.
Regulator of metabolism
Solution
Coenzyme A acts as an acyl group carrier, facilitating the transport of fatty acids into the mitochondria for oxidation.
Correct Answer:
B
— Acyl group carrier
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